Anti-NKCC1 (SLC12A2) Antibody | Alomone Labs ANT-071 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NKCC1 (SLC12A2) Antibody

catalog :

ANT-071

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:100; loading ...; fig 3e	Li Q, Aalling N, Förstera B, Erturk A, Nedergaard M, Møllgård K, et al. Aquaporin 1 and the Na+/K+/2Cl- cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system. Fluids Barriers CNS. 2020;17:15 pubmed publisher
	Smith T, Vasilakos G, Shaffer S, Puglise J, Chou C, Barton E, et al. Novel γ-sarcoglycan interactors in murine muscle membranes. Skelet Muscle. 2022;12:2 pubmed publisher
	Moriguchi S, Kita S, Fukaya M, Osanai M, Inagaki R, Sasaki Y, et al. Reduced expression of Na+/Ca2+ exchangers is associated with cognitive deficits seen in Alzheimer's disease model mice. Neuropharmacology. 2018;131:291-303 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat lung lysate (lanes 3 and 6): - 1-3. Anti-NKCC1 (SLC12A2) (extracellular) Antibody (#ANT-071), (1:200).4-6. Anti-NKCC1 (SLC12A2) (extracellular) Antibody, preincubated with NKCC1/SLC12A2 (extracellular) Blocking Peptide (#BLP-NT071).

image 2 :

Expression of NKCC1 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NKCC1 (SLC12A2) (extracellular) Antibody (#ANT-071), (1:100). A. NKCC1 staining (red) is detected in the cerebellar molecular layer (Mol). B. DAPI counterstain (blue) reveals the outline of cerebellar layers. C. Merge of the two images.

image 3 :

Peptide (C)RRQAMKEMSIDQAK corresponding to amino acid residues 828-841 of human NKCC1 (AccessionP55011). 6thextracellular loop.

product information

CAT :

ANT-071

SKU :

ANT-071-CF_0.2 ml

Product Name :

Anti-NKCC1 (SLC12A2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P55011

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT071

Homology :

Rat - identical; mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RRQAMKEMSIDQAK, corresponding to amino acid residues 828-841 of human NKCC1

Product Page - Scientific background :

The Na-K-Cl cotransporters are a class of membrane proteins that transport Na+, K+, and Cl- ions into and out of cells in an electrically neutral manner, in most cases with a stoichiometry of 1Na+:1K+:2Cl-. Na-K-Cl cotransporters are present in a wide variety of cells and tissues, including reabsorptive and secretory epithelia, nerve and muscle cells, endothelial cells, fibroblasts, and blood cells.Na-K-Cl cotransport plays a vital role in renal salt reabsorption and in salt secretion by intestinal, airway, salivary gland, and other secretory epithelia.1 Na-K-Cl cotransport activity is inhibited by "loop" diuretics, including the clinically efficacious agents, bumetanide and furosemide. The regulation of Na-K-Cl cotransport is mediated, at least in some cases, through direct phosphorylation of the cotransport protein.2NKCC1 is one of two Na-K-Cl cotransporter isoforms that have been identified. NKCC1 is present in a wide variety of secretory epithelia and non-epithelial cells.3 NKCC1 is expressed in virtually all mammalian cells and is thought to play a housekeeping role in cell volume homeostasis and the common control of cytosolic ion content. NKCC1 does not use ATP but operates using electrochemical gradient for Na+ and K+ produced by Na+-K+-ATPase.4 There is considerable evidence that uptake of Cl− in immature neurons is mediated by NKCC1. High expression of NKCC1 in immature neurons plays an important role in maintaining high intracellular Cl−.5

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Na+-K+-2Cl- Cotransporter 1, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, BSC2, Solute carrier family 12 member 2

Short Description :

A Rabbit Polyclonal Antibody to NKCC1 Transporter

Long Description :

Anti-NKCC1 (SLC12A2) Antibody (#ANT-071) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NKCC1 from rat, mouse, and human samples.

Negative Control :

BLP-NT071

Positive Control :

Synonyms :

Na+-K+-2Cl- Cotransporter 1, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, BSC2, Solute carrier family 12 member 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC12A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage