antibody

Anti-CD38 (extracellular) Antibody

ANT-068

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

western blot, immunohistochemistry

ANT-068-CF_0.2 ml

Anti-CD38 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P56528

IFC IHC LCI WB

Rat Mouse

Rabbit

BLP-NT068

Rat - identical The antibody will not recognize human CD38

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, C-terminus.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)KSKHLAHQYTWIQGK, corresponding to amino acid residues 131 - 145 of mouse CD38

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC1, also known as CD38, is a member of the ADP-ribosyl cyclase family. CD38 can act as an enzyme with NAD-depleting and intracellular signaling activity or as a receptor with adhesive functions.1,2CD38, composed of 300 amino acids, is a single chain glycoprotein with a single transmembrane segment that can behave topologically as either a type II or type III membrane protein depending on its orientation within the membrane. CD38 functions as an enzyme that utilizes nicotinamide adenine dinucleotide (NAD+) as a substrate and catalyzes the synthesis of nicotinamide (NAM) and ADPR. NAD+ is an essential cofactor that regulates energy metabolism and can be converted to cADPR, releasing NAM. CD38 can also hydrolyze cADPR to ADP-ribose. These enzymatic functions are significant as both ADPR and cADPR serve as second messengers that regulate various cellular processes by inducing Ca2+ mobilization.1,2In addition to its enzymatic activity, CD38 can function as a receptor for CD31, allowing it to act as an adhesion molecule that mediates selectin-like binding of hematopoietic cells to endothelial cells and facilitates their migration to tissues.1,2CD38 is found in various tissues, with high levels of expression in hematopoietic tissues such as the bone marrow and lymph nodes. In immune cells, it has been detected in B cells, macrophages, dendritic cells, innate lymphoid cells, natural killer cells, T cells, neutrophils, and monocytes. CD38 has been shown to play a role in regulating the release of cytokines, cell adhesion, and cellular migration towards areas of inflammation.1,2,3CD38 is also found in non-hematopoietic tissues, including prostatic epithelial cells, pancreatic islet astrocytes, smooth muscle cells, retinal tubes, kidney, gut, and brain.1,2In the context of the brain, CD38 is expressed in various cell types, including neurons and glial cells. Its role in the central nervous system is multifaceted, and research suggests that it may be involved in processes such as neurotransmission, synaptic plasticity, and neuroinflammation.4As mentioned above, CD38 is a major NAD+-consuming enzyme. NAD has emerged as a key player in energy metabolism, serving as a coenzyme in the production of adenosine triphosphate (ATP) through processes like glycolysis and oxidative phosphorylation. Declines in NAD levels have been observed with aging, and this reduction may impact cellular energy production and contribute to age-related decline in various tissues.4,5 In accordance to that, mice lacking CD38 show increased NAD+ concentrations in the brain, liver, and muscles. Furthermore, due to their resulting enhanced energy expenditure and higher metabolic rates, these animals are protected against obesity, despite a high-fat diet. 4,5In the brain, CD38 expression and activity increases during aging or neuroinflammation conditions, which contributes significantly to age-related NAD+ depletion and mitochondrial dysfunction. In fact, Alzheimer disease pathology is significantly attenuated in mice lacking CD38. 5,6Based on the key role of CD38 in the immune system and the regulation of the immune response, as well as its central role as a regulator of metabolism, through maintenance of NAD+ homeostasis, it is unsurprising that it has emerged as a pharmacological target in various disease contexts, particularly in the fields of cancer, neurodegenerative diseases, and autoimmune disorders.

IFC IHC LCI WB

Alomone Labs

ADP-Ribosyl Cyclase 1, ADPRC 1, Cyclic ADP-Ribose Hydrolase 1, cADPR hydrolase 1, CADPR1

A Rabbit Polyclonal antibody to CD38 (extracellular)

Anti-CD38 (extracellular) Antibody (#ANT-068) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD38 from mouse and rat samples. The antibody will not recognize human samples.

BLP-NT068

NA

ADP-Ribosyl Cyclase 1, ADPRC 1, Cyclic ADP-Ribose Hydrolase 1, cADPR hydrolase 1, CADPR1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

CD38

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:400