Anti-CD157/BST1 (extracellular) Antibody | Alomone Labs ANT-067 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CD157/BST1 (extracellular) Antibody

catalog :

ANT-067

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6):

image 2 :

Western blot analysis of mouse TK-1 T-cell lymphoma cell line lysate (lanes 1 and 4), mouse J774 macrophage cell line lysate (lanes 2 and 5) and mouse WEHI B-cell lymphoma cell line lysate (lanes 3 and 6):

image 3 :

Expression of CD157 in rat deep cerebellar nuclei.

product information

CAT :

ANT-067

SKU :

ANT-067-CF_0.2 ml

Product Name :

Anti-CD157/BST1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q64277

Applications :

IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT067

Homology :

Mouse, Rat - 15 out of 16 amino acid residues identical Not recommended for BST1 from human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KVGDFLSW(S)RQENASG, corresponding to amino acid residues 129 - 144 of mouse BST1

Product Page - Scientific background :

CD157, also known as bone marrow stromal cell antigen 1 (BST1), is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein with a dual function as a receptor and a β-NAD+-metabolizing ectoenzyme of the ADP-ribosyl cyclases gene family1.CD157 and its paralog CD38, catalyze the formation of cyclic ADP-ribose (cADPR) from NAD, a second messenger involved in intracellular calcium mobilization through ryanodine receptors, among other functions 1,2.As a receptor, CD157 interacts with β1 and β2 integrins (CD29 and CD18 respectively) to form a multimolecular complex that mediates intracellular signaling through the FAK/Src pathway 1-3. Known ligands for CD157 include extracellular matrix components like fibronectin, fibrinogen, laminin-1 and type 1 collagen 2,3.As it name suggests, CD157 was first identified in bone marrow-derived cells, but it is also expressed in other immune system cells like neutrophils, T-cells, pre B-cells and macrophages. CD157 has been demonstrated to be involved in both the innate and adaptive immune response with roles in B- and T-cell growth and the control of myeloid cells migration during inflammation 1.CD157 is considered a marker and therapeutic target for several hematological malignancies like acute myeloid leukemia (AML) as well as several solid tumors like ovarian and malignant mesothelioma 3,4,5.Outside the immune system, CD157 is expressed in vascular endothelium, intestine and brain among others. Interestingly, CD157, has been identified as a risk factor for neurodegenerative diseases, particularly Parkinson's disease 2,6. The exact role of CD157 in the nervous system is not clear, but it appears to have a role in neurogenesis and neural stem cells proliferation, as well as a role in neuroinflammation probably through its association with CD18 and CD11b 2,7.Finally, studies with CD157 knockout mice, reveal its involvement in depression-like behaviors, social avoidance and anxiety disorders 2,8.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Bone Marrow Stromal Antigen 1, BST-1, ADP-Ribosyl Cyclase 2, Cyclic ADP-ribose hydrolase 2, CADPr Hydrolase 2, CADPR2, LY-65

Short Description :

A Rabbit Polyclonal antibody to C3aR1 (extracellular)

Long Description :

Anti-CD157/BST1 (extracellular) Antibody (#ANT-067) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD157 from mouse and rat samples. The antibody is not recommended for work with human samples.

Negative Control :

BLP-NT067

Positive Control :

Synonyms :

Bone Marrow Stromal Antigen 1, BST-1, ADP-Ribosyl Cyclase 2, Cyclic ADP-ribose hydrolase 2, CADPr Hydrolase 2, CADPR2, LY-65

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

BST1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage