Anti-CD73 (extracellular) Antibody | Alomone Labs ANT-066 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CD73 (extracellular) Antibody

catalog :

ANT-066

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Esteban P, Garcia Ovejero D, Paniagua Torija B, Moreno Luna R, Arredondo L, Zimmer A, et al. Revisiting CB1 cannabinoid receptor detection and the exploration of its interacting partners. J Neurosci Methods. 2020;337:108680 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1,2. Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200).3,4. Anti-CD73 (extracellular) Antibody, preincubated with CD73 (extracellular) Blocking Peptide (#BLP-NT066).

image 2 :

Expression of CD73 in mouse striatum and hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CD73 staining (green) in mouse striatum is detected in striatal cells (arrows). B. CD73 staining (green) in hippocampal CA1 region is detected in cells of the pyramidal layer (vertical arrow) and their apical dendrites (horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-066

SKU :

ANT-066-CF_0.2 ml

Product Name :

Anti-CD73 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61503

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT066

Homology :

Human, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73

Product Page - Scientific background :

CD73 (ecto-5'-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E

Short Description :

A Rabbit Polyclonal Antibody to CD73

Long Description :

Anti-CD73 (extracellular) Antibody (#ANT-066) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NT5E from human, rat and mouse samples.

Negative Control :

BLP-NT066

Positive Control :

Synonyms :

Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NT5E

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage