Anti-CD39 (extracellular)-FITC Antibody | Alomone Labs ANT-065-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CD39 (extracellular)-FITC Antibody

catalog :

ANT-065-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of CD39 in live intact mouse BV-2 microglia cells: Cell surface detection of CD39 in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-CD39 (extracellular)-FITC Antibody (#ANT-065-F), (5 µg).

product information

CAT :

ANT-065-F

SKU :

ANT-065-F_50 mcl

Product Name :

Anti-CD39 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49961

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT065

Homology :

Mouse - 12/14 amino acid residues identical; rat - 11/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KTSYAGVKEKYLSE, corresponding to amino acid residues 398-411 of human CD39

Product Page - Scientific background :

Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase1, ENTPD1, or CD39) is an enzyme that catalyzes the phosphohydrolysis of extracellular adenosine triphosphate (eATP) and diphosphate (eADP), that are released following inflammatory stress and cell injury, to adenosine monophosphate (AMP). AMP is then used by the ecto-5'-nucleotidase CD73 to synthesize adenosine1.Hydrolysis of adenosine triphosphate and adenosine diphosphate, performed by CD39, suppresses the immune system and inhibits T-cell and Natural killer (NK) cell responses. This inhibition has a role in promoting Chronic lymphocytic leukemia (CLL) and hence it is proposed that blockade or inhibition of CD39 may be a target for new immune therapy for CLL2. CD39 activity is also related to the occurrence of calcific aortic valve disease (CAVD)3, and to various other types of immune responses4. In addition, CD39 plays a dominant role in the purinergic regulation of inflammation1.The transcription of CD39 is constitutive in microvascular endothelium and certain immune cells. The modulated expression of NTPDase1 has been closely associated with inflammatory cytokines, oxidative stress and hypoxia in vitro and in vivo5.Localization of CD39 within lipid rafts suggests that this ecto-enzyme may be involved in cell-cell contact and signaling6.CD39 is a part of the ectonucleoside triphosphate dyphosphohydrolases (eNTDPase) protein family which shares a unique structural feature: instead of being anchored in the membrane by a single transmembrane domain or lipid link like other ectoenzymes, these proteins contain two transmembrane domains, one at each end of the protein. The active site lies in the large extracellular region. In addition, all family members share a set of short sequences termed apyrase conserved regions (ACRs)7.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Ectonucleoside Triphosphate Diphosphohydrolase 1, Ecto-ATP diphosphohydrolase 1, Lymphoid cell activation antigen, ENTPD1

Short Description :

A Rabbit Polyclonal Antibody to CD39 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-CD39 (extracellular) Antibody (#ANT-065) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize ENTPD1 from rat, mouse, and human samples. Anti-CD39 (extracellular)-FITC Antibody (#ANT-065-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Ectonucleoside Triphosphate Diphosphohydrolase 1, Ecto-ATP diphosphohydrolase 1, Lymphoid cell activation antigen, ENTPD1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ENTPD1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-065), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage