Anti-SLC28A3/CNT3 (extracellular) Antibody | Alomone Labs ANT-063 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC28A3/CNT3 (extracellular) Antibody

catalog :

ANT-063

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Jagannath A, Varga N, Dallmann R, Rando G, Gosselin P, Ebrahimjee F, et al. Adenosine integrates light and sleep signalling for the regulation of circadian timing in mice. Nat Commun. 2021;12:2113 pubmed publisher

image

image 1 :

Western blot analysis of mouse kidney membranes (lanes 1 and 5), rat pancreas lysates (lanes 2 and 6), mouse brain membranes (lanes 3 and 7) and rat brain lysate (lanes 4 and 8): - 1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:400). 5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063).

product information

CAT :

ANT-063

SKU :

ANT-063-CF_0.2 ml

Product Name :

Anti-SLC28A3/CNT3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8VIH3

Applications :

IFC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT063

Homology :

Mouse - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

5th extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

Peptide (C)KITLKNAMKMENGDSR, corresponding to amino acid residues 432 - 447 of rat SLC28A3/CNT3

Product Page - Scientific background :

Solute carrier family 28 member 3 (SLC28A3, CNT3) is a sodium-dependent, concentrative pyrimidine- and purine-selective transporter. SLC28A3 plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs, in a process that is coupled to the sodium cellular gradient1.CNT3 consists of 13 putative trans-membrane domains and it forms a homo-trimer which is important for its activity as a transporter2,3.SLC28 members are crucial determinants in the response to a variety of anticancer and antiviral nucleoside analogs, as they modulate the entry of these analogs into target tissues. Within this large group of solute carriers, SLC28A is a sub-group which consists of SLC28A1, SLC28A2, and SLC28A3. Of the three members of this sub-family, SLC28A3 has the broadest selectivity and the widest expression profile4.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3

Short Description :

A Rabbit Polyclonal Antibody to SLC28A3/CNT3

Long Description :

Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize CNT3 from human, rat, and mouse samples.

Negative Control :

BLP-NT063

Positive Control :

Synonyms :

Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC28A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400-1:500

more info or order :

Alomone Labs product webpage