antibody

Anti-PMAT (SLC29A4) Antibody

ANT-054

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ANT-054-CF_0.2 ml

Anti-PMAT (SLC29A4) Antibody

Antibodies

Antibodies

Polyclonal

Q8R139

IHC WB

Human Rat Mouse

Rabbit

BLP-NT054

Rat - identical, Human - 13 out of 15 amino acids identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, 3rd loop.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)GVYMRFDVPRPRVKR, corresponding to amino acid residues 315 - 329 of mouse SLC29A4

Plasma membrane monoamine transporters (PMAT) is a monoamine transporter which has functional and structural similarities to the organic cation transporters (OCT) family1,2. PMAT first cloned in 2004 from the human brain. PMAT transporter belongs to the equilibrative nucleoside transporter (ENT) family but it functions as a polyspecific organic cation transporter3. PMAT (ENT4) has a low-affinity and high-capacity for monoamines 3,4. It was identified as a sodium-independent, electrogenic cation transporter with a wide range of substrates. The protein contains 530 amino acids with 11 transmembrane domains, very similar to the organic cation transporters (OCT) family. A peculiarity of this transporter is its ability to function bidirectionally 1,2,4. PMAT is highly expressed in the brain and skeletal muscle and also found in the liver, kidney, intestine and heart. It efficiently transports many organic cations such as 1-methyl-4-phenylpyridinium (MPP+), which is also the case for OCTs. In particular, PMAT transports histamine with a low affinity but efficiently (high Vmax) 4,5. Similarly, both PMAT and OCTs are sensitive to decynium-22 blockade 4,6. However, PMAT was insensitive to the steroid corticosterone, unlike OCTs 3. Direct comparison of transport efficiency (Vmax/Km) between human OCT3 and PMAT expressed in HEK cells found that 5-HT and dopamine were preferentially taken up by PMAT. Alternatively, OCT3 has a higher preference for histamine, noradrenaline and adrenaline over other biogenic amines 7. The same study found that PMAT was insensitive to inhibitors of high-affinity monoamine transporters such as citalopram, GBR-12909 and desipramine. An earlier study substantiated this by showing a range of antidepressant and antipsychotic drugs had IC50's of 10-20µM at the human PMAT8. PMAT distribution has been investigated in mouse and rat brain9,10. PMAT reported to be widely distributed throughout the brain and not necessarily associated with monoamine pathways. Indeed, it was completely absent from the rat substantia nigra. Expression of PMAT has been reported to be primarily neuronal. Cells double labeled with glial fibrillary acidic protein and PMAT were not observed in any great number in the mouse brain9. However, there is evidence PMAT expression in cultured human astrocytes where they mediate histamine transport11.

IHC WB

Alomone Labs

Plasma Membrane Monoamine Transporter, Equilibrative nucleoside transporter 4, ENT4, Solute carrier family 29 member 4

A Rabbit Polyclonal antibody to PMAT (SLC29A4)

Anti-PMAT (SLC29A4) Antibody (#ANT-054) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMAT from rat, mouse and human samples.

BLP-NT054

NA

Plasma Membrane Monoamine Transporter, Equilibrative nucleoside transporter 4, ENT4, Solute carrier family 29 member 4

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC29A4

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:1000

1:400