Anti-ENT3 (SLC29A3) Antibody | Alomone Labs ANT-053 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ENT3 (SLC29A3) Antibody

catalog :

ANT-053

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat liver lysate (lanes 1 and 4), mouse liver lysate (lanes 2 and 5) and rat kidney membranes (lanes 3 and 6): - 1-3. Anti-ENT3 (SLC29A3) Antibody (#ANT-053), (1:200).4-6. Anti-ENT3 (SLC29A3) Antibody, preincubated with ENT3/SLC29A3 Blocking Peptide (#BLP-NT053).

product information

CAT :

ANT-053

SKU :

ANT-053-CF_0.2 ml

Product Name :

Anti-ENT3 (SLC29A3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80WK7

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT053

Homology :

Mouse - identical; human - 10/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DYPAPGLQRPEDR, corresponding to amino acid residues 37-49 of rat ENT3

Product Page - Scientific background :

Equilibrative nucleoside transporter 3 (ENT3) is responsible for transporting nucleotide synthesis precursors, such as hydrophilic nucleosides and nucleobases within cells1. ENT3 structure includes 11 transmembrane domains and a 51-residue hydrophilic N-terminal region containing two dileucine motifs that are characteristic of endosomal/lysosomal targeting sequences1,2.ENT3 is encoded by the SLC29A3 gene and is expressed in several mouse and human tissues such as placenta, heart, neurons, and astrocytes. In addition, human ENT3 is predominantly expressed in mitochondria in human hepatocytes. Human ENTs are high-capacity transporters, bidirectional, Na+-independent with a wide substrate selectivity1,2. The human ENT family consists of four members: ENT1- ENT4. Nucleoside transport function of ENT3 is maximal at pH 5.5 suggesting that the protein is localized to acidic intracellular compartments; probably lysosomes1,2.Studies show that mutations in human ENT3 can cause various clinical disorders including H syndrome, pigmented hypertrichosis with insulin-dependent diabetes mellitus syndrome, lysosomal storage-like disorders and mitochondrial disorders1,2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Equilibrative nucleoside transporter 3

Short Description :

A Rabbit Polyclonal Antibody to Equilibrative Nucleoside Transporter 3 (SLC29A3)

Long Description :

Anti-ENT3 (SLC29A3) Antibody (#ANT-053) is a highly specific antibody directed against an epitope of the rat equilibrative nucleoside transporter 3. The antibody can be used in western blot analysis. It has been designed to recognize ENT3 from rat, mouse, and human samples.

Negative Control :

BLP-NT053

Positive Control :

Synonyms :

Equilibrative nucleoside transporter 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC29A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage