Anti-ENT1 (SLC29A1) Antibody | Alomone Labs ANT-051 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ENT1 (SLC29A1) Antibody

catalog :

ANT-051

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Van Laar V, Arnold B, Howlett E, Calderon M, St Croix C, Greenamyre J, et al. Evidence for Compartmentalized Axonal Mitochondrial Biogenesis: Mitochondrial DNA Replication Increases in Distal Axons As an Early Response to Parkinson's Disease-Relevant Stress. J Neurosci. 2018;38:7505-7515 pubmed publisher
Vieira C, Ferreirinha F, Magalhães Cardoso M, Silva I, Marques P, Correia de Sá P. Post-inflammatory Ileitis Induces Non-neuronal Purinergic Signaling Adjustments of Cholinergic Neurotransmission in the Myenteric Plexus. Front Pharmacol. 2017;8:811 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney lysates (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1,2. Anti-ENT1 (SLC29A1) Antibody (#ANT-051), (1:200).3,4. Anti-ENT1 (SLC29A1) Antibody, preincubated with ENT1/SLC29A1 Blocking Peptide (#BLP-NT051).

image 2 :

Expression of ENT1 in rat DRG - Immunohistochemical staining of rat frozen dorsal root ganglion (DRG) using Anti-ENT1 (SLC29A1) Antibody (#ANT-051), (1:100), followed by goat anti-rabbit-AlexaFluor- 555 secondary antibody. A. ENT1 labeling (red) appears in the cell bodies of the DRG. Note that the nerve fibers are not stained. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A+B.

image 3 :

Expression of ENT1 in rat DRG - Immunohistochemical staining of rat frozen dorsal root ganglion (DRG) usingAnti-ENT1 (SLC29A1) Antibody (#ANT-051) (1:100) followed by goat anti-rabbit-AlexaFluor- 555 secondary antibody. A. ENT1 labeling (red) appears in the cell bodies of the DRG. Note that the nerve fibers are not stained. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A+B.

product information

CAT :

ANT-051

SKU :

ANT-051-CF_0.2 ml

Product Name :

Anti-ENT1 (SLC29A1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q99808

Applications :

IF IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT051

Homology :

Mouse, rat - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EQETKLDLISKGEEPR, corresponding to amino acid residues 245-260 of human ENT1

Product Page - Scientific background :

Nucleosides play other important roles beyond their nucleic acid synthesis building block role. For example, they are involved in energy metabolism; they serve as ligands of purinergic receptors and act as influential signaling molecules1. Being hydrophilic, nucleosides cannot simply diffuse across the plasma membrane in order to exert their various functions, but rather need to be physically transported via nucleoside transporters1,2.Two different transporter families are responsible for transporting nucleosides across the plasma membrane: The Concentrative Nucleoside Transporter proteins (CNT, SLC28 family), which consist of three members, CNT1-3, and act as Na+-dependent symporters1,3, and the Equilibrative Nucleoside Transporter proteins ENT1-4 (ENT, SLC29 family), which mediate Na+-independent facilitated diffusion. ENTs act as bidirectional carriers, responsible for the influx and efflux of substrates1.Structurally, ENT transporters have eleven transmembrane domains with an intracellular N-terminal and an extracellular C-terminal1.The best characterized ENT transporters are ENT1 and ENT2, which, although display a broader range of substrate selectivity, have lower affinities for nucleosides compared to concentrative transporters. They are ubiquitously expressed. For example, ENT1 is expressed in erythrocytes, vascular endothelium, the placenta, brain, heart, liver and colon1,4. ENT2 displays more or less the same expression pattern but in addition, is strongly expressed in skeletal muscle1,5. ENT3 is a lysosomal pH-dependent transporter capable of transporting adenine, and ENT4 also transports adenine at acidic pH. They are also broadly expressed with ENT3 displaying high expression in the placenta and ENT4 in the heart6,7.As mentioned above, nucleosides have a variety of cellular/physiological functions suggesting that transporters responsible for their trafficking may also have functional attributes. Indeed, ENT1 plays a role in proliferation and therefore is responsible for the constitutive trafficking of nucleosides1.There is no evidence that nucleoside transporters are directly involved in pathophysiologies, but they are clinically significant. For example, nucleoside transporters are responsible for the cellular uptake of a number of nucleoside-derived anticancer drugs1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Equilibrative nucleoside transporter 1, Nucleoside transporter es-type

Short Description :

A Rabbit Polyclonal Antibody to Equilibrative Nucleoside Transporter 1 (SLC29A1)

Long Description :

Anti-ENT1 (SLC29A1) Antibody (#ANT-051) is a highly specific antibody directed against an epitope of the human equilibrative nucleoside transporter 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ENT1 from rat, mouse, and human samples.

Negative Control :

BLP-NT051

Positive Control :

Synonyms :

Equilibrative nucleoside transporter 1, Nucleoside transporter es-type

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC29A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage