Anti-CART Antibody | Alomone Labs ANT-047 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CART Antibody

catalog :

ANT-047

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):

image 2 :

Expression of CART in rat parietal cortex.

image 3 :

Expression of CART in mouse parietal cortex.

product information

CAT :

ANT-047

SKU :

ANT-047-CF_0.2 ml

Product Name :

Anti-CART Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56388

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT047

Homology :

Mouse, rat, human - 13 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Secreted

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CDAGEQ(S)AVRKGAR, corresponding to amino acid residues 95 - 108 of mouse CARTPT

Product Page - Scientific background :

CART peptide, known by the name CARTPT - Cocaine-And Amphetamine-Regulated Transcript Protein. CART peptide is a satiety factor closely associated with the actions of leptin and neuropeptide Y. This anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y (NPY) and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro1.CART peptide is a widely distributed neurotransmitter expressed in the central nervous systems, with a role in reward and reinforcement, feeding, development, sensory processing, stress, and endocrine control. Expression of CART peptide dominates in the mesocorticolimbic dopaminergic (DA) system that extends from the ventral tegmental area (VTA) to the nucleus accumbens (NAc) and includes other limbic areas (amygdala, hippocampus, and frontal cortex), and is also widely distributed in the central nervous system (CNS)2.CART peptide exerted inhibitory effect on psychostimulant-enhanced dopamine receptors signaling, Ca2+/calmodulin-dependent kinase signaling and crucial transcription factors expression. Besides modulation of dopamine receptors-related pathways, CART peptide also exhibited elaborated interactions with other neurotransmitter receptors, such as glutamate receptors and γ-aminobutyric acid receptors, which further account for attribution of CART peptide to inhibition of psychostimulant-potentiated locomotor activity. CART peptide has been shown to have anxiolytic functions on the aversive mood and uncontrolled drug-seeking behaviors following drug withdrawal. Moreover, microinjection of CART peptide has been shown to have an anti-depressant effect, which suggests its potential utility in the mood regulation and avoidance of depression-like behaviors3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Cocaine-And Amphetamine-Regulated Transcript Protein, CARTPT, CART Prepropeptide

Short Description :

A Rabbit Polyclonal antibody to CART peptide

Long Description :

Anti-CART Antibody (#ANT-047) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CART peptide from rat, mouse and human samples.

Negative Control :

BLP-NT047

Positive Control :

Synonyms :

Cocaine-And Amphetamine-Regulated Transcript Protein, CARTPT, CART Prepropeptide

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CARTPT

AB Specifictiy :

The antibody will recognize the precursor (proCART) and both active CART peptides: CART (55-102) and CART (62-102). The human counterpart called CART (42-89) is identical to CART (55-102).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage