Anti-IGF1 Antibody | Alomone Labs ANT-046 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-IGF1 Antibody

catalog :

ANT-046

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis with Anti-IGF1 antibody: - 1, 4. Recombinant rat IGF-I protein (#I-200), 100 ng. 2, 5. Recombinant human IGF1, 100 ng. 3, 6. Recombinant human IGF2, 100 ng. Lanes 1-3: Anti-IGF1 Antibody (#ANT-046), (1:200). Lanes 4-6: Anti-IGF1 Antibody, preincubated with IGF1 Blocking Peptide (BLP-NT046). Note that the antibody recognizes IGF1 (rat and human), but fails to recognize the closely related IGF2 protein.

image 2 :

Expression of IGF1 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-IGF1 Antibody (#ANT-046), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA1 region showed IGF1 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with IGF1 Blocking Peptide (BLP-NT046), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-046

SKU :

ANT-046-CF_0.2 ml

Product Name :

Anti-IGF1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08025

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT046

Homology :

Mouse - identical; Human - 11 out of 12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Secreted

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NKPTGYGSSIRR, corresponding to amino acid residues 74 - 85 of rat IGF1

Product Page - Scientific background :

IGF-1 originally called somatomedin C is a 70-amino acid hormone with homology to IGF-2 and proinsulin. IGF-1 acts as an endocrine, paracrine, and autocrine hormone and is known as a mediator of prenatal and postnatal growth. IGF-1 is mainly synthesized in the liver and regulated by growth hormones (GH) in peripheral tissues such as muscle, cartilage, bone, kidney, nerves, skin, lungs, and the liver itself. The synthesized IGF-1 binds to IGF-1 binding proteins (IGFBPs) with high affinity, IGFBPs regulates the interactions of IGF-1 and its receptor (IGF-1R)1. Binding of IGF-1 to his receptor results in autophosphorylation of the receptor leading to activation of the insulin receptor substrate 1 (IRS1) protein. The activated receptor contains several phosphotyrosine sites that serve as docking sites for many signaling proteins. The association of these proteins together start a complex signaling pathway including the canonical PI3-kinase and mitogen-activated protein (MAP) kinase pathways2.The IGF-1 signaling pathway is a major regulator of the aging process, from rodents to humans3. IGF-1 deficiency follows an autosomal mode of transmission and different mutations can lead to either IGF-1 deletion or low levels of circulation IGF-1. Studies demonstrate IGF-1 deficit can lead to deregulated lipid metabolism, cardiovascular disease (CVD), diabetes, and altered metabolic profile of diabetic patients4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Insulin Like Growth Factor 1, Insulin-Like Growth Factor I, IGF-I, Somatomedin-C

Short Description :

A Rabbit Polyclonal antibody to IGF1

Long Description :

Anti-IGF1 Antibody (#ANT-046) is a highly specific antibody directed against an epitope of the mature rat IGF1 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IGF1 from rat, mouse and human samples. The antibody is specific for IGF1; it does not crossreact with IGF2.

Negative Control :

BLP-NT046

Positive Control :

Synonyms :

Insulin Like Growth Factor 1, Insulin-Like Growth Factor I, IGF-I, Somatomedin-C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

IGF1

AB Specifictiy :

The antibody is specific for IGF1; it does not crossreact with IGF2.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage