antibody

Anti-Human TNF Receptor I (extracellular) Antibody

ANT-041

polyclonal

domestic rabbit

nonconjugated

NA

ANT-041-CF_0.2 ml

Anti-Human TNF Receptor I (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P19438

IFC LCI WB

Human

Rabbit

BLP-NT041

The antibody recognizes only human TNFR1

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

CRKNQYRHYWSEN, corresponding to amino acid residues 127-139 of human TNFR1

Tumor necrosis factor (TNF) has a wide range of biological effects in host defense against pathogens. It is capable of inducing cell survival, proliferation and differentiation as well as both apoptotic and necrotic cell death under certain conditions. The TNFR1 receptor belongs to a family of type I transmembrane proteins.TNFR1, as well as other members of its family, is an elongated molecule composed of an extracellular domain of multiple ~40-residue pseudo repeats, with six cysteines forming three disulfide bridges. These modules are termed CRDs (cysteine-rich domains).Unlike many receptors, TNFR1 does not have enzymatic activity. Instead, an oligomeric complex, called the death domain (DD) places the intracellular regions of the receptor in proximity for recruitment of signaling proteins with enzymatic activities to amplify the signal transduction and mediate extrinsic signal induced cell death1.TNFα, a ligand of TNFR1 binds to the intracellular death domain of TNFR1 and recruits the TNF receptor-associated DD protein (TRADD), which in turn recruits receptor-interacting protein kinase 1 (RIP1), cellular inhibitor of apoptosis proteins 1 and 2 (cIAP1 and 2), and TNF receptor-associated factor 22.TNFR1-mediated signaling provides potent anti-viral activity, however many viruses have evolved to encode and express molecules that specifically inhibit almost every step of TNFR-induced apoptosis and NF-κB signaling pathways3.

IFC LCI WB

Alomone Labs

Tumor necrosis factor receptor 1, TNFR1, TNFRSF1A, TNF receptor superfamily member 1A, CD120a

A Rabbit Polyclonal Antibody to TNF Receptor 1

Anti-Human TNF Receptor I (extracellular) Antibody (#ANT-041) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and can thus detect the protein in living cells. It has been designed to recognize TNFR1 from human samples only.

BLP-NT041

NA

Tumor necrosis factor receptor 1, TNFR1, TNFRSF1A, TNF receptor superfamily member 1A, CD120a

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

TNFRSF1A

This antibody will also recognize the soluble form of human TNF Receptor 1 (also known as Tumor Necrosis Factor Binding Protein 1).

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200