Anti-TROY (TNFRSF19) (extracellular) Antibody | Alomone Labs ANT-033 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TROY (TNFRSF19) (extracellular) Antibody

catalog :

ANT-033

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4): - 1,2. Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033), (1:400).3,4. Anti-TROY (TNFRSF19) (extracellular) Antibody, preincubated with TROY/TNFRSF19 (extracellular) Blocking Peptide (#BLP-NT033).

product information

CAT :

ANT-033

SKU :

ANT-033-CF_0.2 ml

Product Name :

Anti-TROY (TNFRSF19) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JLL3

Applications :

IC IF IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT033

Homology :

Rat, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CRPHRFKEDWGFQK, corresponding to amino acid residues 75-88 of mouse TROY

Product Page - Scientific background :

Tumor necrosis factor (TNF) is a potent mediator of inflammation as well as many normal physiological functions in homeostasis and health, and anti-microbial immunity.The TNF receptor superfamily member 19 (also known as TROY, TNFRSF19) is a type I cell surface transmembrane protein with disulfide bonds that form "cysteine-rich domains" (CRDs). These 40 amino acid pseudo-repeats are defined by 3 intra-chain disulfides generated by 6 highly conserved cysteines1. Unlike other members of the TNF receptor superfamily, TROY lacks a cytoplasmic death domain but contains a single TNF receptor-associated factor (TRAF)-binding site2. TROY is expressed on migrating or proliferating progenitor cells of the hippocampus, thalamus and cerebral cortex.TROY is involved in the pathogenesis of various neoplasms. Glioblastoma multiforme (GBM) is the most malignant form of all primary adult brain tumors in which patient survival remains approximately 1 year. Overexpression of TROY in glioma cells activates Rac1 signaling in a Pyk2-dependent manner to drive glioma cell invasion and migration. TROY expression has been found to correlate inversely with overall patient survival in glioblastoma3.TROY is also involved in the lack of successful axon regeneration in the adult central nervous system. Inhibition of axonal growth arises from inhibitory molecules in CNS myelin, which signal through a common receptor complex on neurons consisting of the ligand-binding Nogo-66 receptor (NgR) and two transmembrane co-receptors, p75NTR and LINGO-1. TROY can substitute p75 and form a tri-receptor complex with NgR and LINGO-1. This complex can reconstitute the activation of RhoA, a required step in the signaling pathways of myelin inhibition. Additionally, both overexpression of a truncated form of TROY lacking its intracellular domain and addition of a TROY-Fc fusion protein can block neuronal response to myelin inhibitors in a dominant-negative manner4.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

TNF receptor superfamily member 19, Toxicity and JNK inducer, TAJ, TRADE

Short Description :

A Rabbit Polyclonal Antibody to TROY

Long Description :

Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize TROY from human, mouse, and rat samples.

Negative Control :

BLP-NT033

Positive Control :

Synonyms :

TNF receptor superfamily member 19, Toxicity and JNK inducer, TAJ, TRADE

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TNFRSF19

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage