Anti-LINGO-1 (extracellular) Antibody | Alomone Labs ANT-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LINGO-1 (extracellular) Antibody

catalog :

ANT-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Geraghty N, Belfiore L, Ly D, Adhikary S, Fuller S, Varikatt W, et al. The P2X7 receptor antagonist Brilliant Blue G reduces serum human interferon-? in a humanized mouse model of graft-versus-host disease. Clin Exp Immunol. 2017;190:79-95 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:200).3,4. Anti-LINGO-1 (extracellular) Antibody, preincubated with LINGO-1 (extracellular) Blocking Peptide (#BLP-NT032).

image 2 :

Expression of LINGO-1 in rat brain and spinal cord - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:400), followed by donkey-anti-rabbit-Cy3. A. LINGO-1 staining (red) is detected in sections of rat substantia nigra, in nigral neurons (arrows). B. Staining of rat spinal cord shows LINGO-1 labeling in ventral horn neurons (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-032

SKU :

ANT-032-CF_0.2 ml

Product Name :

Anti-LINGO-1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9D1T0

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT032

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HVRSYSPDWPHQPNK, corresponding to amino acid residues 507-521 of mouse LINGO-1

Product Page - Scientific background :

LINGO-1 (LRR and Ig domain-containing, nogo receptor interacting protein) is a protein highly expressed in the brain but not in other tissues. There are currently two other known human paralogs- LINGO-2 and LINGO-3. LINGO-1 contains 12 leucine-rich repeat (LRR) motifs flanked by N- and C- terminal capping domains, one immunoglobulin (Ig) domain, a transmembrane domain and a short cytoplasmic tail. The cytoplasmic tail contains a canonical epidermal growth factor receptor-like tyrosine phosphorylation site. LINGO-1 is highly conserved evolutionary with human and mouse orthologues, sharing 99.5% identity. LINGO-1 binds both to Nogo-66 receptor (NgR1) and to p75 neurotrophin receptor simultaneously and can form a ternary complex1.In animal models, LINGO-1 expression is upregulated in rat spinal cord injury, experimental autoimmune encephalomyelitis, neurotoxic lesions and glaucoma. In humans, LINGO-1 expression is increased in oligodendrocyte progenitor cells from demyelinated white matter of multiple sclerosis post-mortem samples, and in dopaminergic neurons from Parkinson's disease brains. LINGO-1 negatively regulates oligodendrocyte differentiation and myelination, neuronal survival and axonal regeneration by activating the RhoA and inhibiting protein kinase B (Akt) phosphorylation signaling pathways2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat neuronal protein 6A, LRRN6A, LERN1

Short Description :

A Rabbit Polyclonal Antibody to LINGO-1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse LINGO-1. Anti-LINGO-1 (extracellular) Antibody (#ANT-032) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting the protein in living cells. It has been designed to recognize LINGO-1 from human, mouse, and rat samples.

Negative Control :

BLP-NT032

Positive Control :

Synonyms :

Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat neuronal protein 6A, LRRN6A, LERN1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LINGO1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage