Anti-CNTF Antibody | Alomone Labs ANT-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CNTF Antibody

catalog :

ANT-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis using Anti-CNTF Antibody on: - 1. Recombinant human CNTF protein (#C-240), 20ng.2. Recombinant human Cardiotrophin-1 protein (#C-200), 20ng.3. Recombinant human LIF protein (#L-200), 20ng.4. mIL-6, 20 ng.5. Recombinant human CNTF protein (#C-240), 20ng.Lanes 1-4. Anti-CNTF Antibody (#ANT-031), (1:200).Lane 5. Anti-CNTF Antibody, preincubated with CNTF Blocking Peptide (#BLP-NT031).Antibody specificity is demonstrated in that it specifically recognizes CNTF while it does not reacts with the related cytokines Cardiotrophin-1, LIF and IL-6.

image 2 :

Expression of Ciliary neurotrophic factor (CNTF) in rat hippoccampus - Immunohistochemical staining of rat hippoccampus using Anti-CNTF Antibody (#ANT-031), (1:200). A. CNTF staining (green) reveals astrocyte-profiles (arrows) in the hilus (H) of the dentate gyrus. B. Staining the same section with the astrocyte marker, anti-glial fibrillary acidic protein (red), visualizes astrocytes in the hilus. C. Merge of the two images shows that CNTF is indeed expressed in astrocytes.

image 3 :

Expression of CNTF in rat C6 glioma cells - Immunocytochemical staining of fixed and permeabilized rat C6 glioma cells. A. Cells were stained withAnti-CNTF Antibody (#ANT-031) (1:200) followed by goat anti-rabbit-DyLight-594 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.

product information

CAT :

ANT-031

SKU :

ANT-031-CF_0.2 ml

Product Name :

Anti-CNTF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P26441

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT031

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF

Product Page - Scientific background :

CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR)5.CNTF can support the survival of many different cell populations within the PNS and the CNS6. in vitro, CNTF promotes proliferation and neuronal specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons7, induces sprouting of cholinergic motor neurons8 and delays neural degeneration in genetic models of motor neuron disease9. In addition, it is involved in the development stage of astrocytes and oligodendocytes10.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Ciliary neurotrophic factor

Short Description :

A Rabbit Polyclonal Antibody to CNTF

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CNTF. Anti-CNTF Antibody (#ANT-031) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CNTF from human, rat and mouse samples. Anti-CNTF Antibody specifically recognizes CNTF and not related cytokines.

Negative Control :

BLP-NT031

Positive Control :

Synonyms :

Ciliary neurotrophic factor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNTF

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage