Anti-MANF/ARMET Antibody | Alomone Labs ANT-028 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MANF/ARMET Antibody

catalog :

ANT-028

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Dong L, Hu Y, Zhou L, Cheng X. P2X7 receptor antagonist protects retinal ganglion cells by inhibiting microglial activation in a rat chronic ocular hypertension model. Mol Med Rep. 2018;17:2289-2296 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human recombinant MANF protein (lanes 3 and 6): - 1-3. Anti-MANF/ARMET Antibody (#ANT-028), (1:200).4-6. Anti-MANF/ARMET Antibody, preincubated with MANF/ARMET Blocking Peptide (#BLP-NT028).

image 2 :

Expression of MANF in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-MANF/ARMET Antibody (#ANT-028), (1:600), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Sections derived from parietal cortex of a rat at postnatal day 22 reveal MANF staining (green) in nuclei and cytoplasm of several cells (arrows) in deep layers of the cortex. Nuclei are stained with DAPI (blue).

image 3 :

Expression of MANF in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-MANF/ARMET Antibody(#ANT-028) (1:600) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Sections derived from parietal cortex of a rat at postnatal day 22 reveal MANF staining (green) in nuclei and cytoplasm of several cells (arrows) in deep layers of the cortex. Nuclei are stained with DAPI (blue).

product information

CAT :

ANT-028

SKU :

ANT-028-CF_0.2 ml

Product Name :

Anti-MANF/ARMET Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P55145

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT028

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Mature MANF protein

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF

Product Page - Scientific background :

The novel protein mesencephalic astrocyte-derived neurotrophic factor (MANF) is a survival molecule (alternatively referred to as arginine-rich, mutated in early stage tumors, or ARMET) that has high selectivity for dopaminergic neurons of the midbrain1,2. There, it induces proliferative and protective effects against neurodegeneration (usually caused by 6-hydroxydopamine); its therapeutic potential in dopamine-related neurodegenerative disorders such as Parkinson's disease is therefore promising3,4.MANF is also expressed cardiac tissues. As was recently suggested, it induces cardioprotection in the heart and anti-hypertrophic effects via reducing endoplasmic reticulum stress in ischemic conditions in both the heart and the brain. Thus, its effects stretch beyond rescue and protection of dopaminergic neurons alone1,5.Generally, neurotrophic factors (NTFs) provide support, protection, and regulation for neurons - with each group of neurons having their own unique NTFs - both during development and at adulthood. Other noticeable NTFs include neurotrophin-3 and -4 (NT3 and NT4), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), and glial cell derived neurotrophic factor (GDNF)6.MANF is considered to be evolutionary conserved within Mammalia and beyond (including distant species such as nematode and fruit fly - the latter shares more than 50% identity with human MANF gene) and is highly mutative in many tumors. It is mostly composed of alpha helices and random coils; its structure essentially differs from any other neurotrophic factor. It is suggested that the protein initiates intracellular signaling cascade by binding to cell-surface lipids and membranes via its cysteine-rich N-terminal. Together with its C-terminal which contains a disulfide bridge that endows a cytoprotective response against ER stress, MANF is implied to be bi-functional3,7.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP

Short Description :

A Rabbit Polyclonal Antibody to MANF

Long Description :

Anti-MANF/ARMET Antibody (#ANT-028) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MANF from human, rat and mouse samples.

Negative Control :

BLP-NT028

Positive Control :

Synonyms :

Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MANF

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage