Anti-CDNF Antibody | Alomone Labs ANT-027 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CDNF Antibody

catalog :

ANT-027

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of 100 ng human recombinant CDNF (lanes 1 and 3) and 100 ng human recombinant MANF (lanes 2 and 4): - 1,2. Anti-CDNF Antibody (#ANT-027) (1:500).3,4. Anti-CDNF Antibody, preincubated with CDNF Blocking Peptide (#BLP-NT027). Note that the antibody recognizes CDNF but fails to recognize closely related MANF.

image 2 :

Expression of CDNF in mouse brain - Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using rabbit Anti-CDNF Antibody (#ANT-027), (1:300). A. CDNF staining (green) is detected in several cells in the vicinity of the substantia nigra pars compacta. B. Tyrosine hydroxylase staining (red) appears in the substantia nigra. C. Merge of the two images demonstrates dopaminergic (TH-positive) neurons also expressing CDNF (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-027

SKU :

ANT-027-CF_0.2 ml

Product Name :

Anti-CDNF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q49AH0

Applications :

IF IHC WB

Reactivity :

Human Rat

Host :

Rabbit

Blocking Peptide :

BLP-NT027

Homology :

Mouse - 10/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Mature CDNF protein

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EKTLDLASVDLRK, corresponding to amino acid residues 129-141 of human CDNF

Product Page - Scientific background :

Neurotropic factors (NTFs) comprise an important group of secreted proteins regulating the life cycle of neurons during development. NTFs prevent apoptosis and control the number of neurons innervating a target tissue. NTFs also regulate migration, differentiation and maturation of postmitotic neuronal precursors and promote the regeneration of neurons after injury.Cerebral dopamine neurotrophic factor (CDNF) is a member of a sub-family of NTF proteins. The premature form of the protein consists of 187 amino acids while the mature form contains 161 residues. CDNF has a saposin-like N-terminal domain and an unstructured carboxy C-terminal domain with an intradomain cysteine bridge in a CXXC motif. Notably, saposin-like proteins interact with lipids, suggesting that CDNF's N-terminal may also interact with lipids. The saposin-like domain of CDNF crystalizes as a dimer at pH 4.6. It is presumed that CDNF functions upon synthesis and secretion since it does not contain prosequences for enzymatic activation.When CDNF is injected into the striatum of rats after the injection of 6-OHDA (6-OHDA leads to protracted retrograde degeneration of the nigrostriatal pathway) it significantly reduces amphetamine-induced rotational behavior and protects tyrosine hydroxylase (TH) positive dopaminergic cell bodies in the SN and TH-positive fibers in the striatum. In addition, CDNF had showed neurorestorative effects in the same animal model.Currently it is still unknown if CDNF also affects other types of neurons in the CNS or whether it has any effect on the PNS. It is also not clear to which target molecule CDNF binds and what signaling pathways it activates. CDNF might serve as a therapeutic agent for neurodegenerative diseases1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Cerebral dopamine neurotrophic factor, Arginine-rich mutated in early stage tumors-like 1, ARMET-like protein 1, ARMETL1

Short Description :

A Rabbit Polyclonal Antibody to Cerebral Dopamine Neurotrophic Factor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CDNF. Anti-CDNF Antibody (#ANT-027) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CDNF from mouse and human samples. The antibody will not recognize CDNF from rat samples.

Negative Control :

BLP-NT027

Positive Control :

Synonyms :

Cerebral dopamine neurotrophic factor, Arginine-rich mutated in early stage tumors-like 1, ARMET-like protein 1, ARMETL1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CDNF

AB Specifictiy :

Not recommended for rat samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage