Anti-proGDNF Antibody | Alomone Labs ANT-026 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-proGDNF Antibody

catalog :

ANT-026

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Izumi Y, Wakita S, Kanbara C, Nakai T, Akaike A, Kume T. Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons. Sci Rep. 2017;7:42111 pubmed publisher

image

image 1 :

Western blot analysis of human GDNF pro-domain fusion protein at 40 ng (lanes 1 and 4), 30 ng (lanes 2 and 5) and 20ng (lanes 3 and 6): - 1-3. Anti-proGDNF Antibody (#ANT-026), (1:200).4-6. Anti-proGDNF Antibody, preincubated with proGDNF Blocking Peptide (#BLP-NT026). Note that the expected MW of the GDNF pro-domain fusion protein is 21kD.

image 2 :

Peptide (C)PEDYPDQFDDVMD corresponding to amino acid residues 54-66 of human GDNF (precursor) (AccessionP39905). Pro-domain of the GDNF protein.

product information

CAT :

ANT-026

SKU :

ANT-026-CF_0.2 ml

Product Name :

Anti-proGDNF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P39905

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT026

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Pro-domain of the GDNF protein

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)PEDYPDQFDDVMD, corresponding to amino acid residues 54-66 of human GDNF (precursor)

Product Page - Scientific background :

Glial-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily. GDNF signals through a multi-component receptor system, composed of a RET protooncogene and one of the four α1-α4 receptors1.GDNF promotes survival of various neuronal cells, including motoneurons2,3, Purkinje cells and sympathetic neurons4. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake5. Cells that express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells6, neurons, pinealocytes, and skeletal muscle cells7.In vivo, following transection of facial motor neuron axons, locally applied GDNF has been shown to rescue virtually all damaged neurons from death8. GDNF may be of clinical relevance in the treatment of Parkinson's disease that is characterized by progressive degeneration of midbrain dopaminergic neurons9,10.Recently, it has been hypothesized that functional, carboxy-terminally amidated peptides are processed from the GDNF precursor upon proteolytic cleavage by furin-like endopeptidase11,12,13. Those different peptides (a 5-mer and 11-mer) have not been isolated endogenously to date. However, the rat 11-mer sequence (named brain excitatory peptide, BEP) significantly induced synaptic excitability and possessed some dopaminergic activities in vitro (thus named dopamine neuron stimulating peptides, DNSP)13. Furthermore, the human 11-mer sequence (named DNSP-11) exhibits neurotrophic-like properties13.Thus, the role of the full proDomain of GDNF, which is a product of proteolytic cleavage of proGDNF, is not clearly understood yet.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Glial cell line-derived neurotrophic factor precursor

Short Description :

A Rabbit Polyclonal Antibody to proGDNF

Long Description :

Anti-proGDNF Antibody (#ANT-026) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize proGDNF from human, mouse, and rat samples. The antibody is specific for the pro-domain region of GDNF and will not react with mature GDNF.

Negative Control :

BLP-NT026

Positive Control :

Synonyms :

Glial cell line-derived neurotrophic factor precursor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GDNF

AB Specifictiy :

The antibody is specific for the pro-domain region of GDNF and will not react with mature GDNF.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage