Anti-GFR alpha 4 (extracellular) Antibody | Alomone Labs ANT-024 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GFR alpha 4 (extracellular) Antibody

catalog :

ANT-024

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:800).3,4. Anti-GFR alpha 4 (extracellular) Antibody, preincubated with GFR α 4 (extracellular) Blocking Peptide (#BLP-NT024).

image 2 :

Expression of GFRA4 in rat red nucleus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:100). Staining reveals expression of GFRA4 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).

image 3 :

Peptide (C)GSRDG(S)PEEGGPR corresponding to amino acid residues 160-172 of rat GFRA4 (AccessionQ9EPI2). Extracellular N-terminus.

product information

CAT :

ANT-024

SKU :

ANT-024-CF_0.2 ml

Product Name :

Anti-GFR alpha 4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9EPI2

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT024

Homology :

Rat - 12/13 amino acid residues identical; mouse - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFRA4

Product Page - Scientific background :

The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2.These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1.In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

GFRA4, GFRα4, GDNF family receptor α4, GDNF receptor alpha 4, Persephin receptor

Short Description :

A Rabbit Polyclonal Antibody to GDNF Family Receptor α4

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GDNF receptor α4. Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024) can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GFR α4 from mouse and rat samples. The antibody is not suitable for detecting GFR α4 from human samples.

Negative Control :

BLP-NT024

Positive Control :

Synonyms :

GFRA4, GFRα4, GDNF family receptor α4, GDNF receptor alpha 4, Persephin receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GFRA4

AB Specifictiy :

Not recommended for human samples. The antibody will recognize both the membrane bound canonical isoform and the putative secreted isoform.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage