Anti-GFR alpha 1 (extracellular) Antibody | Alomone Labs ANT-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GFR alpha 1 (extracellular) Antibody

catalog :

ANT-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021), (1:200).3,4. Anti-GFR alpha 1 (extracellular) Antibody, preincubated with GFR α 1 (extracellular) Blocking Peptide (#BLP-NT021).

image 2 :

Expression of GFRA1 in rat neocortex - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021), (1:100). A. GFRA1 (green) is visualized in neocortex neurons. B. Neurons expressing γ-amino butyric acid (GABA) are labeled with parvalbumin (red). C. Merge of the two images demonstrates partial colocalization (arrows).

image 3 :

Expression of GFRA1 in live intact rat C6 glioma cells - Cell surface detection ofGFRA1 in live intact rat C6 glioma cells with Anti-GFR alpha 1 (extracellular) Antibody(#ANT-021) (1:50) followed by goat-anti-rabbit-DyLight-594 secondary antibody (red) (A). B. Live view of the cells. C. Merge of the two images.

product information

CAT :

ANT-021

SKU :

ANT-021-CF_0.2 ml

Product Name :

Anti-GFR alpha 1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62997

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT021

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRSAMEALKQKS, corresponding to amino acid residues 72-83 of rat GFRA1

Product Page - Scientific background :

The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2.These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1.In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3. In respect to their important role in development, individual knockout of either gdnf, gfra1 or ret gives rise to lethal phenotypes2.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

GFRA1, GFRα1, GDNF family receptor α1, GDNF receptor alpha 1, Ret ligand 1, RETL1, TRNR1

Short Description :

A Rabbit Polyclonal Antibody to GDNF Family Receptor α1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GDNF receptor α1. Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021) can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GFR α1 from mouse, rat and human samples.

Negative Control :

BLP-NT021

Positive Control :

Synonyms :

GFRA1, GFRα1, GDNF family receptor α1, GDNF receptor alpha 1, Ret ligand 1, RETL1, TRNR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GFRA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage