Anti-Neurotensin Receptor 2 Antibody | Alomone Labs ANT-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurotensin Receptor 2 Antibody

catalog :

ANT-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Blondy S, Talbot H, Saada S, Christou N, Battu S, Pannequin J, et al. Overexpression of sortilin is associated with 5-FU resistance and poor prognosis in colorectal cancer. J Cell Mol Med. 2021;25:47-60 pubmed publisher
Talbot H, Saada S, Barthout E, Gallet P, Gachard N, Abraham J, et al. BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells. Sci Rep. 2020;10:12572 pubmed publisher
Abbaci A, Talbot H, Saada S, Gachard N, Abraham J, Jaccard A, et al. Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis. Oncogene. 2017;: pubmed publisher

image

image 1 :

Western blot analysis of rat dorsal root ganglion lysates: - 1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).

image 2 :

Expression of NTR2 in rat mediobasal hypothalamus - Immunohistochemical staining of perfusion-fixed brain frozen rat mediobasal (MB) hypothalamus sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:100), (green). A. NTR2 appears in neuronal fibers marked by white arrows. B. In the same section, there is staining for glial fibrillary acidic protein (red fluorescence), a marker of astrocytes. C. Merging the two images demonstrates that NTR2 is not localized in astrocytes. DAPI is used as the counterstain (blue).

image 3 :

Western blot analysis of mouse brain lysates: - 1.Anti-Neurotensin Receptor 2 Antibody (#ANT-016) (1:400).2. Anti-Neurotensin Receptor 2 Antibody preincubated with the control peptide antigen.

product information

CAT :

ANT-016

SKU :

ANT-016-CF_0.2 ml

Product Name :

Anti-Neurotensin Receptor 2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63384

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT016

Homology :

Mouse - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CGEQHSLVPLPQEAPE, corresponding to amino acid residues 377-392 of rat NTSR2

Product Page - Scientific background :

Neurotensin receptor 2 (NTR2) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.NTR2 and NTR1 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.1NTR2 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+mobilization. However, the exact signaling mechanisms subsequent to NTR2 activation appear to depend on the species and on the cellular system in which the receptor is studied.NTR2 is expressed mainly in the central nervous system and dorsal root ganglion (DRG) neurons but has been also observed in peripheral tissues such as gastric parietal cells where it probably mediates the neurotensin-induced effects on gastric acid secretion.2The most established physiological role of NTR2 is its role in pain transmission as NTR2-defficient mice showed that NTR2 is the likely mediator of the neurotensin-induced analgesic responses.3

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

NTR2, NTSR2, Levocabastine-sensitive neurotensin receptor

Short Description :

A Rabbit Polyclonal Antibody to Neurotensin Receptor 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope at the intracellular C-terminal domain of the rat Neurotensin receptor type 2. Anti-Neurotensin Receptor 2 Antibody (#ANT-016) can be used in western blot, immunohistochemical and immunocytochemical applications, and recognizes NTR2 from rat and mouse samples.

Negative Control :

BLP-NT016

Positive Control :

Synonyms :

NTR2, NTSR2, Levocabastine-sensitive neurotensin receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NTSR2

AB Specifictiy :

Unlikely to recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:100

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage