Anti-Neurotensin Receptor 1 (extracellular) Antibody | Alomone Labs ANT-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurotensin Receptor 1 (extracellular) Antibody

catalog :

ANT-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Popov J, Bandura J, Markovic F, Borojevic R, Anipindi V, Pai N, et al. Influence of bacterial components on the developmental programming of enteric neurons. Physiol Rep. 2020;8:e14611 pubmed publisher

image

image 1 :

Western blot analysis of human colon cancer HT-29 (lanes 1 and 4), human lung small cell carcinoma NCI-H526 (lanes 2 and 5) and human breast adenocarcinoma MDA-MB-468 (lanes 3 and 6) cell lysates: - 1-3. Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:200).4-6. Anti-Neurotensin Receptor 1 (extracellular) Antibody, preincubated with Neurotensin Receptor 1 (extracellular) Blocking Peptide (#BLP-NT015).

image 2 :

Expression of NTSR1 in human breast cancer - Immunohistochemical staining of human breast cancer cells using Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:100). Staining (brown color) is specific for epithelium-derivated malignant cells. Hematoxilin is used as the counterstain.

image 3 :

Cell surface detection ofNTSR1 in live intact human promyelocytic leukemia HL-60 cell line: - ___Cells + FITC-conjugated goat anti rabbit antibody.___Cells +Anti-Neurotensin Receptor 1 (extracellular)Antibody (#ANT-015) (1:20) + FITC-conjugated goat-anti-rabbit antibody.

product information

CAT :

ANT-015

SKU :

ANT-015-CF_0.2 ml

Product Name :

Anti-Neurotensin Receptor 1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30989

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT015

Homology :

Mouse - 14/16 amino acid residues identical; rat - 12/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

EQNRSADGQHAGGLVC corresponding to amino acid residues 209-224 of human NTSR1

Product Page - Scientific background :

Neurotensin receptor 1 (NTR1) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.NTR1 and NTR2 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.1NTR1 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+ mobilization, although coupling with Gi/o and to Gs has been observed in some expression systems.NTR1 is mainly expressed in the brain and gastrointestinal tract. In the brain, NTR1 mediates body temperature regulation, feeding behavior and locomotion regulation. One of the best studied roles of NTR1 is the regulation of dopamine release suggesting that NTR1 can be a suitable target for the development of a new class of anti-psychotics.2 In the periphery, NTR1 expression has been linked to the control of intestine motility and in the stimulation of growth of the intestine mucosa in both physiological and pathological conditions. NTR1 has been shown to be upregulated in several human cancers where it stimulates tumor growth in response to locally produced neurotensin peptide.3

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR

Short Description :

A Rabbit Polyclonal Antibody to Neurotensin Receptor 1

Long Description :

Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015) is a highly specific antibody directed against an epitope of the human NTSR1. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and flow cytometry applications. It has been designed to recognize NTSR1 from human, mouse, and rat samples.

Negative Control :

BLP-NT015

Positive Control :

Synonyms :

NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NTSR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage