Anti-GDNF Antibody | Alomone Labs ANT-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GDNF Antibody

catalog :

ANT-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Izumi Y, Wakita S, Kanbara C, Nakai T, Akaike A, Kume T. Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons. Sci Rep. 2017;7:42111 pubmed publisher

image

image 1 :

Western blot analysis of Recombinant human GDNF protein: - Lanes 1, 4: 50 ng Recombinant human GDNF protein (#G-240).Lanes 2, 5: 10 ng Recombinant human GDNF protein.Lanes 3, 4: 1 ng Recombinant human GDNF protein.Lanes 1-3: Anti-GDNF Antibody (#ANT-014), (1:1000).Lanes 4-5: Anti-GDNF Antibody, preincubated with GDNF Blocking Peptide (#BLP-NT014).

image 2 :

Expression of GDNF in rat dorsal root ganglion (DRG). - Immunohistochemical staining of rat DRG longitudinal frozen section using Anti-GDNF Antibody (#ANT-014), (1:50), followed by Alexa 555-conjugated goat anti-rabbit antibody (red). Hoechst 33342 (blue) was used as a counterstain. GDNF staining is specific for neurons. Note that Schwann cells as well as satellite glial cells are both negative.

image 3 :

Peptide (C)DDNLVYHILRKHSAKR corresponding to amino acid residues 192-207 of human GDNF (precursor) (AccessionP39905). C-terminus of the mature GDNF.

product information

CAT :

ANT-014

SKU :

ANT-014-CF_0.2 ml

Product Name :

Anti-GDNF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P39905

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT014

Homology :

Mouse, dog, monkey, bovine - identical; rat 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

C-terminus of the mature GDNF

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor)

Product Page - Scientific background :

Glial cell-line derived neurotrophic factor (GDNF) is a neurotrophic factor originally identified for its ability to promote the survival of dopaminergic neurons in the midbrain.1 GDNF is part of the neurotrophic factor family known as GDNF-family ligand (GFL), which includes neurturin (NRTN), artemin (ARTN), and persephin (PSPN).2,3 The GFL ligands are structurally related to the transforming growth factor-b (TGF-b) protein superfamily and contain seven cysteine residues with the same relative spacing as other members of this family.2Typical of neurotrophic factors, GDNF is produced in the form of a precursor, preproGDNF, which is then cleaved during secretion to become proGDNF, and later processed to the mature (homodimeric) GDNF form.2 The GFLs convey their activity by binding to a multicomponent receptor that includes the RET receptor tyrosine kinase and a glycosyl phosphatidylinositol (GPI)-linked ligand-binding subunit known as GDNF family receptor α (GFRα).2,3 Four different GFRα subunits have been identified (GFRα 1-4) that determine the specificity of the GFRα-RET receptor complex. Thus, GFRα1 together with RET make up the high affinity receptor for GDNF. The GDNF and GFRα complex interaction with RET induces activation of the intracellular tyrosine kinase domain of the latter. Phosphorylated RET can then interact with several intracellular signaling cascades including MAP kinase, PI3K and PLCγ signaling pathways.2,3 Lately a RET-independent signaling pathway for GDNF has been shown to involve binding of GDNF and GFRα to the neural cell adhesion molecule (NCAM). Signaling of GDNF via the NCAM complex stimulates intracellular tyrosine kinases such as Fyn and FAK.4GDNF is synthesized and secreted by a wide variety of cell types in the central and peripheral nervous system where it exerts its neurotrophic effects in the development and maintenance of distinct sets of neurons. Accordingly, GDNF knockout mice die shortly after birth, reflecting the central role of GDNF in neurodevelopment.2 Remarkably, GDNF was found to be an essential morphogen for kidney formation as well as to have a role is spermatogenesis.3

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Glial-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor

Short Description :

A Rabbit Polyclonal Antibody to GDNF

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human GDNF. Anti-GDNF Antibody (#ANT-014) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize GDNF from human, mouse and rat samples.

Negative Control :

BLP-NT014

Positive Control :

PCP-G240

Synonyms :

Glial-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GDNF

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:100

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage