Anti-proNT-3 Antibody | Alomone Labs ANT-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-proNT-3 Antibody

catalog :

ANT-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis using Anti-proNT-3 Antibody (#ANT-012) (1:600): - 1. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (200 ng).2. Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (200 ng).3. Native mouse NGF 2.5S protein (>95%) (#N-100), (200 ng).4. Recombinant human beta-NGF protein (#N-245), (200 ng).5. Recombinant human BDNF protein (#B-250), (200 ng).6. Recombinant mouse proNGF protein (#N-250), (200 ng).7. Recombinant mouse proBDNF protein (#B-240), (200 ng).8. Recombinant proNT-3, (10 ng).

image 2 :

Expression of proNT-3 in rat brain - Immunohistochemical staining of rat Islands of Calleja (IOC) using Anti-proNT-3 Antibody (#ANT-012). A. proNT-3 staining (green) overlaps neuronal nitric oxide synthase (nNOS red) while a cluster of cells covers a part of the island (DAPI blue counterstain) but not a cell-poor area (star). B. The distribution of proNT-3 is shown alone. Note that staining is more intense in the cell poor area demarcated by white line. C. The distribution of neuronal NOS is most intense in the cell-poor region.

image 3 :

Western blot analysisusing Anti-proNT-3 Antibody (#ANT-012) (1:600): - 1.Recombinant human Neurotrophin-3 (NT-3) protein(#N-260)(200 ng).2.Recombinant human Neurotrophin-4 (NT-4) protein(#N-270) (200 ng).3.Native mouse NGF 2.5S protein ( 95%)(#N-100) (200 ng).4.Recombinant human beta-NGF protein(#N-245) (200 ng).5.Recombinant human BDNF protein(#B-250)(200 ng).6.Recombinant mouse proNGF protein(#N-250)(200 ng).7.Recombinant mouse proBDNF protein(#B-240)(200 ng).8. Recombinant proNT-3 (10 ng).

product information

CAT :

ANT-012

SKU :

ANT-012-CF_0.2 ml

Product Name :

Anti-proNT-3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P20783

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT012

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Pro-domain of the NT-3 protein

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor)

Product Page - Scientific background :

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins. These are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.Until recently, the functional role of the neurotrophin prodomain was thought to assist in the correct folding of the mature protein and the sorting of the neurotrophin into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions. Several studies implicate both proNGF and proBDNF in several physiological and pathological conditions.It remains to be established whether proNT-3 has different biological functions than the mature NT-3 neurotrophin.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

pro-Neurotrophin 3, proNTF3

Short Description :

A Rabbit Polyclonal Antibody to proNT-3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human proNT-3. Anti-proNT-3 Antibody (#ANT-012) can be used in western blot and immunohistochemistry applications. It has been designed to recognize proNT-3 from rat, mouse and human samples. The antibody does not cross reacts with mature NT-3, pro- and mature NGF or pro- and mature BDNF.

Negative Control :

BLP-NT012

Positive Control :

Synonyms :

pro-Neurotrophin 3, proNTF3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NTF3

AB Specifictiy :

The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:300-1:600

more info or order :

Alomone Labs product webpage