Anti-p75 NGF Receptor (intracellular) Antibody | Alomone Labs ANT-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-p75 NGF Receptor (intracellular) Antibody

catalog :

ANT-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Yamamoto N, Hiramatsu N, Ohkuma M, Hatsusaka N, Takeda S, Nagai N, et al. Novel Technique for Retinal Nerve Cell Regeneration with Electrophysiological Functions Using Human Iris-Derived iPS Cells. Cells. 2021;10: pubmed publisher
Salvo E, Tu N, Scheff N, Dubeykovskaya Z, Chavan S, Aouizerat B, et al. TNFα promotes oral cancer growth, pain, and Schwann cell activation. Sci Rep. 2021;11:1840 pubmed publisher
Foltran R, Stefani K, Bonafina A, Resasco A, Diaz S. Differential Hippocampal Expression of BDNF Isoforms and Their Receptors Under Diverse Configurations of the Serotonergic System in a Mice Model of Increased Neuronal Survival. Front Cell Neurosci. 2019;13:384 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011), (1:200).2. Anti-p75 NGF Receptor (intracellular) Antibody, preincubated with p75 NGF Receptor Blocking Peptide (#BLP-NT011).

image 2 :

Expression of p75NTR in mouse hippocampus - Immunohistochemical staining of mouse frozen hippocampal sections using Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011) (1:200). A. p75NTR (green) appears in a subset of astrocytes. GFAP (glial fibrillary acidic protein) staining (red) to label astrocytes. C. A merge of the two images shows p75NTR found mainly in stratum radiatum (SOR) and only in a few astrocytes in the alveus (AL).

image 3 :

Western blot analysisof human melanoma cells A875: - 1. Anti-p75NGF Receptor (intracellular) Antibody(#ANT-011) (1:200).2. Anti-p75NGF Receptor (intracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ANT-011

SKU :

ANT-011-CF_0.2 ml

Product Name :

Anti-p75 NGF Receptor (intracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08138

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT011

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, juxtamembrane domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CKQNKQGANSRPVNQT corresponding to amino acid residues 278-293 of human p75NTR

Product Page - Scientific background :

The p75 neurotrophin receptor (p75NTR) is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Short Description :

A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human p75NTR. Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011) can be used in western blot and immunohistochemistry applications. It has been designed to recognize p75NTR from human, mouse and rat samples.

Negative Control :

BLP-NT011

Positive Control :

Synonyms :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGFR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage