Anti-BDNF Antibody | Alomone Labs ANT-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-BDNF Antibody

catalog :

ANT-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat; 1:300; loading ...; fig 4di	Cohen S, Matar M, Vainer E, Zohar J, Kaplan Z, Cohen H. Significance of the orexinergic system in modulating stress-related responses in an animal model of post-traumatic stress disorder. Transl Psychiatry. 2020;10:10 pubmed publisher
	Belity T, Horowitz M, Hoffman J, Epstein Y, Bruchim Y, Todder D, et al. Heat-Stress Preconditioning Attenuates Behavioral Responses to Psychological Stress: The Role of HSP-70 in Modulating Stress Responses. Int J Mol Sci. 2022;23: pubmed publisher
	Fyk Kolodziej B, Mueller P. Sedentary Conditions Promote Subregionally Specific Changes in Brain-Derived Neurotrophic Factor in the Rostral Ventrolateral Medulla. Front Physiol. 2021;12:756542 pubmed publisher
	Vignoli B, Sansevero G, Sasi M, Rimondini R, Blum R, Bonaldo V, et al. Astrocytic microdomains from mouse cortex gain molecular control over long-term information storage and memory retention. Commun Biol. 2021;4:1152 pubmed publisher
	Duarte Silva E, Meiry da Rocha Araújo S, Oliveira W, Lós D, Bonfanti A, Peron G, et al. Sildenafil Alleviates Murine Experimental Autoimmune Encephalomyelitis by Triggering Autophagy in the Spinal Cord. Front Immunol. 2021;12:671511 pubmed publisher
	Elsayed H, Faroni A, Ashraf M, Osuji J, Wunderley L, Zhang L, et al. Development and Characterisation of an in vitro Model of Wallerian Degeneration. Front Bioeng Biotechnol. 2020;8:784 pubmed publisher
	Poddar I, Callahan P, Hernandez C, Pillai A, Yang X, Bartlett M, et al. Oral quetiapine treatment results in time-dependent alterations of recognition memory and brain-derived neurotrophic factor-related signaling molecules in the hippocampus of rats. Pharmacol Biochem Behav. 2020;197:172999 pubmed publisher
	Homa M, Loyens A, Eddarkaoui S, Faivre E, Deramecourt V, Maurage C, et al. The TMEM240 Protein, Mutated in SCA21, Is Expressed in Purkinje Cells and Synaptic Terminals. Cerebellum. 2020;19:358-369 pubmed publisher
	Perez V, Bermedo García F, Zelada D, Court F, Perez M, Fuenzalida M, et al. The p75NTR neurotrophin receptor is required to organize the mature neuromuscular synapse by regulating synaptic vesicle availability. Acta Neuropathol Commun. 2019;7:147 pubmed publisher
	Lambert W, Carlson B, Ghose P, Vest V, Yao V, Calkins D. Towards A Microbead Occlusion Model of Glaucoma for a Non-Human Primate. Sci Rep. 2019;9:11572 pubmed publisher
	Kim D, Cho J, Kang H. Protective effect of exercise training against the progression of Alzheimer's disease in 3xTg-AD mice. Behav Brain Res. 2019;374:112105 pubmed publisher
	Shin M, Choi M, Chae H, Kim J, Kim H, Kim K. Ganglioside GQ1b ameliorates cognitive impairments in an Alzheimer's disease mouse model, and causes reduction of amyloid precursor protein. Sci Rep. 2019;9:8512 pubmed publisher
	Eremenko E, Mittal K, Berner O, Kamenetsky N, Nemirovsky A, Elyahu Y, et al. BDNF-producing, amyloid β-specific CD4 T cells as targeted drug-delivery vehicles in Alzheimer's disease. EBioMedicine. 2019;43:424-434 pubmed publisher
	Escudero C, Cabeza C, Moya Alvarado G, Maloney M, Flores C, Wu C, et al. c-Jun N-terminal kinase (JNK)-dependent internalization and Rab5-dependent endocytic sorting mediate long-distance retrograde neuronal death induced by axonal BDNF-p75 signaling. Sci Rep. 2019;9:6070 pubmed publisher
	Schiller A, Hong J, Xia Z, Wang H. Increased Brain-Derived Neurotrophic Factor in Lumbar Dorsal Root Ganglia Contributes to the Enhanced Exercise Pressor Reflex in Heart Failure. Int J Mol Sci. 2019;20: pubmed publisher
	Yannai S, Zonszain J, Donyo M, Ast G. Combinatorial treatment increases IKAP levels in human cells generated from Familial Dysautonomia patients. PLoS ONE. 2019;14:e0211602 pubmed publisher
	Sun C, KRIMM R, Hill D. Maintenance of Mouse Gustatory Terminal Field Organization Is Dependent on BDNF at Adulthood. J Neurosci. 2018;38:6873-6887 pubmed publisher
	Ignowski E, Winter A, Duval N, Fleming H, Wallace T, Manning E, et al. The cysteine-rich whey protein supplement, Immunocal®, preserves brain glutathione and improves cognitive, motor, and histopathological indices of traumatic brain injury in a mouse model of controlled cortical impact. Free Radic Biol Med. 2018;124:328-341 pubmed publisher
	Yun D, Zhuang Y, Kreutz M, Behnisch T. The role of 19S proteasome associated deubiquitinases in activity-dependent hippocampal synaptic plasticity. Neuropharmacology. 2018;133:354-365 pubmed publisher
	Krizo J, Moreland L, Rastogi A, MOU X, Prosser R, Mintz E. Regulation of Locomotor activity in fed, fasted, and food-restricted mice lacking tissue-type plasminogen activator. BMC Physiol. 2018;18:2 pubmed publisher
	Dragatsis I, Dietrich P, Ren H, Deng Y, Del Mar N, Wang H, et al. Effect of early embryonic deletion of huntingtin from pyramidal neurons on the development and long-term survival of neurons in cerebral cortex and striatum. Neurobiol Dis. 2018;111:102-117 pubmed publisher
	Obradovic A, Atluri N, Dalla Massara L, Oklopcic A, Todorovic N, Katta G, et al. Early Exposure to Ketamine Impairs Axonal Pruning in Developing Mouse Hippocampus. Mol Neurobiol. 2018;55:164-172 pubmed publisher
	Fukuchi M, Izumi H, Mori H, Kiyama M, Otsuka S, Maki S, et al. Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice. Sci Rep. 2017;7:4949 pubmed publisher
	Hoffman J, Zuckerman A, Ram O, Sadot O, Stout J, Ostfeld I, et al. Behavioral and inflammatory response in animals exposed to a low-pressure blast wave and supplemented with Î²-alanine. Amino Acids. 2017;49:871-886 pubmed publisher
	Benhar I, Reemst K, Kalchenko V, Schwartz M. The retinal pigment epithelium as a gateway for monocyte trafficking into the eye. EMBO J. 2016;35:1219-35 pubmed publisher
	Wattananit S, Tornero D, Graubardt N, Memanishvili T, Monni E, Tatarishvili J, et al. Monocyte-Derived Macrophages Contribute to Spontaneous Long-Term Functional Recovery after Stroke in Mice. J Neurosci. 2016;36:4182-95 pubmed publisher

image

image 1 :

Western blot analysis with Anti-BDNF Antibody: - 1,5. Recombinant human BDNF protein (#B-250).2,6. Recombinant mouse proBDNF protein (#B-240).3,7. Recombinant human beta-NGF protein (#N-245).4,8. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260).Lanes 1-4: Anti-BDNF Antibody (#ANT-010), (1:200).Lanes 5-8: Anti-BDNF Antibody, preincubated with BDNF Blocking Peptide (#BLP-NT010). Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.

image 2 :

Expression of BDNF in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-BDNF Antibody (#ANT-010). A. BDNF (red) appears in Purkinje cells (upward pointing arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (downward pointing arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein (green) in the same section demonstrates the distribution of BDNF to neuronal as well as to astrocytic cellular components. C. Confocal merge of BDNF and GFAP.

image 3 :

Peptide (C)GEFGGHDKPKITAWE corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (AccessionO35458). Cytoplasmic N-terminus.

product information

CAT :

ANT-010

SKU :

ANT-010-CF_0.2 ml

Product Name :

Anti-BDNF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23560

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT010

Homology :

Mouse, rat and many other species - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)

Product Page - Scientific background :

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system.Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its "rapid" neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Brain-derived neurotrophic factor

Short Description :

A Rabbit Polyclonal Antibody to BDNF

Long Description :

Anti-BDNF Antibody (#ANT-010) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human, and mouse samples. The antibody is specific for BDNF; it does not crossreact with NGF, NT-3 or NT-4.

Negative Control :

BLP-NT010

Positive Control :

PCP-B250

Synonyms :

Brain-derived neurotrophic factor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

BDNF

AB Specifictiy :

The antibody is specific for BDNF; it does not crossreact with NGF, NT-3 or NT-4.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage