Guinea pig Anti-BDNF Antibody | Alomone Labs ANT-010-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-BDNF Antibody

catalog :

ANT-010-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
T xf3 th K, Oroszi T, Van der Zee E, Nyakas C, Schoemaker R. Effects of exercise training on behavior and brain function after high dose isoproterenol-induced cardiac damage. Sci Rep. 2021;11:23576 pubmed publisher
Duarte Silva E, Meiry da Rocha Araújo S, Oliveira W, Lós D, Bonfanti A, Peron G, et al. Sildenafil Alleviates Murine Experimental Autoimmune Encephalomyelitis by Triggering Autophagy in the Spinal Cord. Front Immunol. 2021;12:671511 pubmed publisher
Kim T, Park S, Park J, Park H. Infusion of Plasma from Exercised Mice Ameliorates Cognitive Dysfunction by Increasing Hippocampal Neuroplasticity and Mitochondrial Functions in 3xTg-AD Mice. Int J Mol Sci. 2020;21: pubmed publisher
Kelly M, Smith D, Sunshine M, Ross A, Zhang X, Gumz M, et al. Circadian clock genes and respiratory neuroplasticity genes oscillate in the phrenic motor system. Am J Physiol Regul Integr Comp Physiol. 2020;318:R1058-R1067 pubmed publisher
Kumar H, Choi H, Jo M, Joshi H, Muttigi M, Bonanomi D, et al. Neutrophil elastase inhibition effectively rescued angiopoietin-1 decrease and inhibits glial scar after spinal cord injury. Acta Neuropathol Commun. 2018;6:73 pubmed publisher

image

image 1 :

Western blot analysis of 100 ng of each Recombinant human BDNF protein (#B-250) (lanes 1 and 5), Recombinant mouse proBDNF protein (#B-240) (lanes 2 and 6), Recombinant human Neurotrophin-3 (NT-3) protein (#N-260) (lanes 3 and 7) and Recombinant human beta-NGF protein (#N-245) (lanes 4 and 8): - 1-4. Guinea pig Anti-BDNF Antibody (#ANT-010-GP), (1:500).5-8. Guinea pig Anti-BDNF Antibody, preincubated with BDNF Blocking Peptide (#BLP-NT010).Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.

image 2 :

Expression of BDNF in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-BDNF Antibody (#ANT-010-GP), (1:400), followed by goat anti-guinea pig-Cy3. BDNF staining (red) in the hippocampal dentate gyrus region, appears in neuronal profiles in the granule layer (G) and in interneurons in the hilus (H) region (arrows). DAPI counterstain is used to visualize nuclei of all cells (blue).

product information

CAT :

ANT-010-GP

SKU :

ANT-010-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-BDNF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23560

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-NT010

Homology :

Mouse, rat and many other species - identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)

Product Page - Scientific background :

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system.Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its "rapid" neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Brain-derived neurotrophic factor

Short Description :

A Guinea Pig Polyclonal Antibody to BDNF

Long Description :

Guinea pig Anti-BDNF Antibody (#ANT-010-GP, formerly AGP-021) is a highly specific antibody directed against an epitope of human brain-derived neurotrophic factor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human and mouse samples. The antibody is specific for BDNF; it does not crossreact with NGF, NT-3 or NT-4. The antigen used to immunize guinea pigs is the same as Anti-BDNF Antibody (#ANT-010) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-NT010

Positive Control :

PCP-B250

Synonyms :

Brain-derived neurotrophic factor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

BDNF

AB Specifictiy :

The antibody is specific for BDNF; it does not crossreact with the closely related neurotrophins NGF, NT-3 or NT-4.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage