Anti-Sortilin (extracellular) Antibody | Alomone Labs ANT-009 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Sortilin (extracellular) Antibody

catalog :

ANT-009

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 2a, 2b	Hu Z, Luo C, Hurtado P, Li H, Wang S, Hu B, et al. Brain-derived neurotrophic factor precursor in the immune system is a novel target for treating multiple sclerosis. Theranostics. 2021;11:715-730 pubmed publisher
	Marsland M, Dowdell A, Jiang C, Wilmott J, Scolyer R, Zhang X, et al. Expression of NGF/proNGF and Their Receptors TrkA, p75NTR and Sortilin in Melanoma. Int J Mol Sci. 2022;23: pubmed publisher
	Tepper B, Bartkowska K, Okrasa M, Ngati S, Braszak M, Turlejski K, et al. Downregulation of TrkC Receptors Increases Dendritic Arborization of Purkinje Cells in the Developing Cerebellum of the Opossum, Monodelphis domestica. Front Neuroanat. 2020;14:56 pubmed publisher
	Faulkner S, Griffin N, Rowe C, Jobling P, Lombard J, Oliveira S, et al. Nerve growth factor and its receptor tyrosine kinase TrkA are overexpressed in cervical squamous cell carcinoma. FASEB Bioadv. 2020;2:398-408 pubmed publisher
	Gao F, Griffin N, Faulkner S, Rowe C, Williams L, Roselli S, et al. The neurotrophic tyrosine kinase receptor TrkA and its ligand NGF are increased in squamous cell carcinomas of the lung. Sci Rep. 2018;8:8135 pubmed publisher
	Al Akhrass H, Naves T, Vincent F, Magnaudeix A, Durand K, Bertin F, et al. Sortilin limits EGFR signaling by promoting its internalization in lung cancer. Nat Commun. 2017;8:1182 pubmed publisher
	Faulkner S, Jobling P, Rowe C, Rodrigues Oliveira S, Roselli S, Thorne R, et al. Neurotrophin Receptors TrkA, p75NTR, and Sortilin Are Increased and Targetable in Thyroid Cancer. Am J Pathol. 2018;188:229-241 pubmed publisher
	Ioannou M, Fahnestock M. ProNGF, but Not NGF, Switches from Neurotrophic to Apoptotic Activity in Response to Reductions in TrkA Receptor Levels. Int J Mol Sci. 2017;18: pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate: - 1,2. Anti-Sortilin (extracellular) Antibody (#ANT-009), (1:400).3,4. Anti-Sortilin (extracellular) Antibody, preincubated with Sortilin (extracellular) Blocking Peptide (#BLP-NT009).

image 2 :

Expression of Sortilin in rat neocortex - Immunohistochemical staining of rat neocortex with Anti-Sortilin (extracellular) Antibody (#ANT-009). A. Sortilin appears in cortical neurons (red). B. Staining of interneurons with mouse anti-parvalbumin (PV, green). C. Confocal merge of Sortilin and PV demonstrates separate localization in neocortex.

image 3 :

Expression of Sortilin in rat neocortex - Immunohistochemical staining of rat neocortex with Anti-Sortilin (extracellular)Antibody (#ANT-009). A. Sortilin appears in cortical neurons (red). B. Staining of interneurons with mouse anti-parvalbumin (PV green). C. Confocal merge of Sortilin and PV demonstrates separate localization in neocortex.

product information

CAT :

ANT-009

SKU :

ANT-009-CF_0.2 ml

Product Name :

Anti-Sortilin (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q99523

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT009

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin

Product Page - Scientific background :

Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has lately been identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in the brain, spinal cord, skeletal muscle and adipocytes.Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90% of sortilin is retained in intracellular compartments with only about 10% reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95

Short Description :

A Rabbit Polyclonal Antibody to Sortilin

Long Description :

Anti-Sortilin (extracellular) Antibody (#ANT-009) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize Sortilin from mouse, rat, and human samples.

Negative Control :

BLP-NT009

Positive Control :

Synonyms :

SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SORT1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage