Anti-Sortilin (extracellular)-FITC Antibody | Alomone Labs ANT-009-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Sortilin (extracellular)-FITC Antibody

catalog :

ANT-009-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Tepper B, Bartkowska K, Okrasa M, Ngati S, Braszak M, Turlejski K, et al. Downregulation of TrkC Receptors Increases Dendritic Arborization of Purkinje Cells in the Developing Cerebellum of the Opossum, Monodelphis domestica. Front Neuroanat. 2020;14:56 pubmed publisher

image

image 1 :

Cell surface detection of Sortilin in live intact mouse J774 macrophage cells: Cell surface detection of Sortilin in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-Sortilin (extracellular)-FITC Antibody (#ANT-009-F), (2.5 µg).

product information

CAT :

ANT-009-F

SKU :

ANT-009-F_50 mcl

Product Name :

Anti-Sortilin (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q99523

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT009

Homology :

Mouse, Rat - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular domain

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin

Product Page - Scientific background :

Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has been lately identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, Sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in brain, spinal cord, skeletal muscle and adipocytes.Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90% of sortilin is retained in intracellular compartments with only about 10% reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Sortilin 1, SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95

Short Description :

A Rabbit Polyclonal Antibody to Sortilin Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Sortilin (extracellular) Antibody (#ANT-009) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize Sortilin from mouse, rat, and human samples. Anti-Sortilin (extracellular)-FITC Antibody (#ANT-009-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Sortilin 1, SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SORT1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-009), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage