Anti-Nogo Receptor (extracellular)-FITC Antibody | Alomone Labs ANT-008-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nogo Receptor (extracellular)-FITC Antibody

catalog :

ANT-008-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Nogo Receptor in live intact mouse BV-2 microglia cells: Cell surface detection of Nogo Receptor in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-Nogo Receptor (extracellular)-FITC Antibody (#ANT-008-F), (2.5 µg).

product information

CAT :

ANT-008-F

SKU :

ANT-008-F_15 mcl

Product Name :

Anti-Nogo Receptor (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9BZR6

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT008

Homology :

Mouse, rat - 15/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor

Product Page - Scientific background :

Neurite outgrowth inhibitor receptor 1 (NgR1, Nogo Receptor) is a glycosylphosphatidylinositol (GPI) transmembrane protein expressed on the surface of axons in the CNS. Its key binding domain is an extracellular leucine-rich repeat domain that interacts with Myelin-associated inhibitory factors (MAIFs) composed of MAG, Neurite outgrowth inhibitor (Nogo)-A, and OMgp1,2.NgR1 binds to Neurite outgrowth inhibitor (Nogo) through a short hydrophilic loop between two hydrophobic domains. This binding activates intracellular molecular switches through the heteromerization with NgR1 co-receptors, p75NTR and LINGO-1.This process facilitates neurite outgrowth dynamics; it also governs the plasticity of the CNS at the axo-dendritic and axo-glial levels3.During CNS neurodegenerative disorders and in animal models of inflammatory demyelination, MAIFs, containing Nogo, accumulate and therefore there is an increased binding to their cognate receptor. This marks Nogo-NgR1 interaction as a potential target of new therapeutics for neurodegenerative diseases4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Short Description :

A Rabbit Polyclonal Antibody to Nogo Receptor Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Nogo Receptor (extracellular) Antibody (#ANT-008) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NgR1 from human, mouse and rat samples. Anti-Nogo Receptor (extracellular)-FITC Antibody (#ANT-008-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

RTN4R

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-008), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage