Anti-p75 NGF Receptor (extracellular) Antibody | Alomone Labs ANT-007 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-p75 NGF Receptor (extracellular) Antibody

catalog :

ANT-007

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; loading ...; fig 3c	Zhang N, Kisiswa L, Ramanujan A, Li Z, Sim E, Tian X, et al. Structural basis of NF-κB signaling by the p75 neurotrophin receptor interaction with adaptor protein TRADD through their respective death domains. J Biol Chem. 2021;297:100916 pubmed publisher
	Clark A, Kugathasan U, Baskozos G, Priestman D, Fugger N, Lone M, et al. An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions. Cell Rep Med. 2021;2:100345 pubmed publisher
	Wong L, Tann J, Ibanez C, Sajikumar S. The p75 neurotrophin receptor is an essential mediator of impairments in hippocampal-dependent associative plasticity and memory induced by sleep deprivation. J Neurosci. 2019;: pubmed publisher
	Escudero C, Cabeza C, Moya Alvarado G, Maloney M, Flores C, Wu C, et al. c-Jun N-terminal kinase (JNK)-dependent internalization and Rab5-dependent endocytic sorting mediate long-distance retrograde neuronal death induced by axonal BDNF-p75 signaling. Sci Rep. 2019;9:6070 pubmed publisher
	Hsu M, Yang M, Schnegg C, Hwang S, Ryu B, Alani R. Notch3 signaling-mediated melanoma-endothelial crosstalk regulates melanoma stem-like cell homeostasis and niche morphogenesis. Lab Invest. 2017;97:725-736 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007), (1:200).2. Anti-p75 NGF Receptor (extracellular) Antibody, preincubated with p75 NGF Receptor (extracellular) Blocking Peptide (#BLP-NT007).

image 2 :

Expression of p75NTR in rat brain - Immunohistochemical staining of rat brain with Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007). A. Cells in the diagonal band are stained positive for p75NTR. B. Staining of the same section with goat anti-ChAT confirms that p75NTR staining is specific to cholinergic neurons.

product information

CAT :

ANT-007

SKU :

ANT-007-CF_0.2 ml

Product Name :

Anti-p75 NGF Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08138

Applications :

IC IF IFC IHC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT007

Homology :

Mouse - identical; rat - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, stalk domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR

Product Page - Scientific background :

The p75 neurotrophin receptor (p75NTR) is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.

Applications may also work in :

IC IF IFC IHC IP LCI WB

Supplier :

Alomone Labs

Target :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Short Description :

A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR

Long Description :

Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize p75NTR from human, mouse and rat samples.

Negative Control :

BLP-NT007

Positive Control :

Synonyms :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGFR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage