Anti-p75 NGF Receptor (extracellular)-FITC Antibody | Alomone Labs ANT-007-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-p75 NGF Receptor (extracellular)-FITC Antibody

catalog :

ANT-007-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Salvo E, Tu N, Scheff N, Dubeykovskaya Z, Chavan S, Aouizerat B, et al. TNFα promotes oral cancer growth, pain, and Schwann cell activation. Sci Rep. 2021;11:1840 pubmed publisher
Escudero C, Cabeza C, Moya Alvarado G, Maloney M, Flores C, Wu C, et al. c-Jun N-terminal kinase (JNK)-dependent internalization and Rab5-dependent endocytic sorting mediate long-distance retrograde neuronal death induced by axonal BDNF-p75 signaling. Sci Rep. 2019;9:6070 pubmed publisher
Hsu M, Yang M, Schnegg C, Hwang S, Ryu B, Alani R. Notch3 signaling-mediated melanoma-endothelial crosstalk regulates melanoma stem-like cell homeostasis and niche morphogenesis. Lab Invest. 2017;97:725-736 pubmed publisher
WILLIAMS K, KILLEBREW D, Clary G, Meeker R. Opposing Effects of NGF and proNGF on HIV Induced Macrophage Activation. J Neuroimmune Pharmacol. 2016;11:98-120 pubmed publisher

image

image 1 :

Cell surface detection of p75NTR in live intact rat glioma C6 cells: Cell surface detection of p75NTR in live intact rat glioma C6 cells: Black line: Unstained cells. Green line: Cells + Anti-p75 NGF Receptor (extracellular)-FITC Antibody (#ANT-007-F). The control antigen is not suitable for this application.

image 2 :

Cell surface detection of p75NTR inlive intact rat glioma C6 cells: - ___Unstained cells.___Cells + Anti-p75NGF Receptor (extracellular)-FITC Antibody(#ANT-007-F).The control antigen is not suitable for this application.

product information

CAT :

ANT-007-F

SKU :

ANT-007-F_50 mcl

Product Name :

Anti-p75 NGF Receptor (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08138

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT007

Homology :

Mouse - identical; rat - 15/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, stalk domain

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR

Product Page - Scientific background :

The p75 neurotrophin receptor (p75NTR) is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Short Description :

A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize p75NTR from human, mouse and rat samples. Anti-p75 NGF Receptor (extracellular)-FITC Antibody (#ANT-007-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGFR

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-007), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage