Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody | Alomone Labs ANT-007-AO product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody

catalog :

ANT-007-AO

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Salvo E, Tu N, Scheff N, Dubeykovskaya Z, Chavan S, Aouizerat B, et al. TNFα promotes oral cancer growth, pain, and Schwann cell activation. Sci Rep. 2021;11:1840 pubmed publisher
Pathak A, Stanley E, Hickman F, Wallace N, Brewer B, Li D, et al. Retrograde Degenerative Signaling Mediated by the p75 Neurotrophin Receptor Requires p150Glued Deacetylation by Axonal HDAC1. Dev Cell. 2018;46:376-387.e7 pubmed publisher

image

image 1 :

Multiplex staining of LINGO-1 and p75NTR in rat hippocampus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:200) and Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody (#ANT-007-AO), (1:80). A. LINGO-1 staining (green) in hippocampal CA3 region is detected in neuronal profiles (arrows). B. p75NTR staining in the same section (orange) is also seen in CA3 neurons (arrows). C. Merge of the two images demonstrates colocalization in several neurons (arrows). Cell nuclei are stained with DAPI (blue).

image 2 :

Cell surface detection of Glucose transporter 3 in live intact mouse J774 macrophage cell line: - ___Cells.___Cells + goat-anti-rabbit-FITC.___Cells +Anti-GLUT3 (extracellular)Antibody (#AGT-023) (5 g) + goat-anti-rabbit-FITC.

product information

CAT :

ANT-007-AO

SKU :

ANT-007-AO_50 mcl

Product Name :

Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08138

Applications :

IC IF IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT007

Homology :

Mouse - identical; rat - 15/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, stalk domain

Label :

ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR

Product Page - Scientific background :

The p75 neurotrophin receptor (p75NTR) is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.

Applications may also work in :

IC IF IHC LCI

Supplier :

Alomone Labs

Target :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Short Description :

A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-550

Long Description :

Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize p75NTR from human, mouse and rat samples. Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody (#ANT-007-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AO

Positive Control :

Synonyms :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGFR

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-007), and immunocytochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage