Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs ANT-007-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody

catalog :

ANT-007-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 4c	Clark A, Kugathasan U, Baskozos G, Priestman D, Fugger N, Lone M, et al. An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions. Cell Rep Med. 2021;2:100345 pubmed publisher
	Yamamoto N, Hiramatsu N, Ohkuma M, Hatsusaka N, Takeda S, Nagai N, et al. Novel Technique for Retinal Nerve Cell Regeneration with Electrophysiological Functions Using Human Iris-Derived iPS Cells. Cells. 2021;10: pubmed publisher

image

image 1 :

Immunolocalization of p75NTR and TrkA in rat dorsal root ganglion - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody (#ANT-007-AG), (1:60) and Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (#ANT-018-FR), (1:60). A. Sections were labeled with Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody (green). B. The same section was stained with Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (purple). C. Merge of the two images suggests extensive co-localization (arrow points at an example) of TrkA and p75NTR receptors in rat dorsal root ganglion. Cell nuclei were stained with DAPI (blue).

image 2 :

Cell surface detection of p75NTR in live intact rat pheochromocytoma (PC12) cells: Cell surface detection of p75NTR in live intact rat pheochromocytoma (PC12) cells: Black line: Unstained cells. Green line: Cells + Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody (#ANT-007-AG), (1:20).

product information

CAT :

ANT-007-AG

SKU :

ANT-007-AG_50 mcl

Product Name :

Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08138

Applications :

FC IC IF IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT007

Homology :

Mouse - identical; rat - 15/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, stalk domain

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR

Product Page - Scientific background :

The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis factor (TNF) receptor superfamily. Like all members of this superfamily, p75NTR is a type I transmembrane protein with tandem cysteine-rich domains in its extracellular portion, as well as an intracellular death domain. p75NTR binds to its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor, and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors determine the ligand being recognized (see below) and the biological response to its binding1-3.As its name implies, p75NTR binds to all the neurotrophins (NTR) (NGF, BDNF, NT-3, and NT-4) with similar nanomolar affinities. Co-expression of p75NTR with the Trk receptors enhances its ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF), probably as a complex with sortilin, leading to cell death by apoptosis1-3. Finally, a multimeric complex of p75NTR and Nogo receptor binds to myelin proteins such as Nogo-66, MAG, and OmgP, resulting in inhibition of axonal growth4.

Applications may also work in :

FC IC IF IHC LCI

Supplier :

Alomone Labs

Target :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Short Description :

A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize p75NTR from human, mouse and rat samples. Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody (#ANT-007-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGFR

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-007), and immunocytochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage