Guinea pig Anti-proBDNF Antibody | Alomone Labs ANT-006-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-proBDNF Antibody

catalog :

ANT-006-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Duarte Silva E, Meiry da Rocha Araújo S, Oliveira W, Lós D, Bonfanti A, Peron G, et al. Sildenafil Alleviates Murine Experimental Autoimmune Encephalomyelitis by Triggering Autophagy in the Spinal Cord. Front Immunol. 2021;12:671511 pubmed publisher
Oliver R, Brigman J, Bolognani F, Allan A, Neisewander J, Perrone Bizzozero N. Neuronal RNA-binding protein HuD regulates addiction-related gene expression and behavior. Genes Brain Behav. 2018;17:e12454 pubmed publisher

image

image 1 :

Western blot analysis of 50 ng of each Recombinant human Val66Met proBDNF (cleavage resistant) protein (#B-456) (lanes 1 and 4), Recombinant mouse proBDNF protein (#B-240) (lanes 2 and 5) and Recombinant human proBDNF protein (#B-257) (lanes 3 and 6): - 1-3. Guinea pig Anti-proBDNF Antibody (#ANT-006-GP), (1:200).4-6. Guinea pig Anti-proBDNF Antibody, preincubated with proBDNF Blocking Peptide (#BLP-NT006).

image 2 :

Expression of proBDNF in rat spinal cord - Immunohistochemical staining of immersion-fixed, free floating rat spinal cord frozen sections using Guinea pig Anti-proBDNF Antibody (#ANT-006-GP), (1:1000). proBDNF (red) is detected in the superficial layer (arrow) of the dorsal horn of the spinal cord. The myelinated columns are devoid of staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-006-GP

SKU :

ANT-006-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-proBDNF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23560

Applications :

IF IHC WB

Reactivity :

Human Rat

Host :

Guinea Pig

Blocking Peptide :

BLP-NT006

Homology :

Mouse, rat - 16/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Pro-domain of the BDNF protein

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor)

Product Page - Scientific background :

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the prodomain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP), a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer's disease.Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Brain-derived neurotrophic factor precursor

Short Description :

A Guinea Pig Polyclonal Antibody to proBDNF

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against the prodomain region of human proBDNF. Guinea pig Anti-proBDNF Antibody (#ANT-006-GP, formerly AGP-032), raised in guinea pig, can be used in western and immunohistochemistry applications. In addition, the antibody is an excellent tool for easy multiplex staining studies. It has been designed to recognize proBDNF from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-proBDNF Antibody (#ANT-006) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-NT006

Positive Control :

PCP-B257

Synonyms :

Brain-derived neurotrophic factor precursor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

BDNF

AB Specifictiy :

The antibody is specific for proBDNF, it does not crossreact with proNGF, proNT-3 or mature BDNF.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage