Anti-proNGF Antibody | Alomone Labs ANT-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-proNGF Antibody

catalog :

ANT-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 10

Reference
Marsland M, Dowdell A, Jiang C, Wilmott J, Scolyer R, Zhang X, et al. Expression of NGF/proNGF and Their Receptors TrkA, p75NTR and Sortilin in Melanoma. Int J Mol Sci. 2022;23: pubmed publisher
Du Z, Song Y, Chen X, Zhang W, Zhang G, Li H, et al. Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor. Aging Cell. 2021;20:e13437 pubmed publisher
Li A, Feng L, Niu X, Zeng Q, Li B, You Z. Downregulation of OIP5-AS1 affects proNGF-induced pancreatic cancer metastasis by inhibiting p75NTR levels. Aging (Albany NY). 2021;13:10688-10702 pubmed publisher
Pentz R, Iulita M, Mikutra Cencora M, Ducatenzeiler A, Bennett D, Cuello A. A new role for matrix metalloproteinase-3 in the NGF metabolic pathway: Proteolysis of mature NGF and sex-specific differences in the continuum of Alzheimer's pathology. Neurobiol Dis. 2021;148:105150 pubmed publisher
Pham D, Bruelle C, Thi Do H, Pajanoja C, Jin C, Srinivasan V, et al. Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells. Cell Death Dis. 2019;10:537 pubmed publisher
Elshaer S, El Remessy A. Deletion of p75NTR prevents vaso-obliteration and retinal neovascularization via activation of Trk- A receptor in ischemic retinopathy model. Sci Rep. 2018;8:12490 pubmed publisher
Ryu J, Tooke K, Malley S, Soulas A, Weiss T, Ganesh N, et al. Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. 2018;128:1772-1786 pubmed publisher
Mohamed R, Coucha M, Elshaer S, Artham S, Lemtalsi T, El Remessy A. Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction. Biochim Biophys Acta Mol Basis Dis. 2018;1864:746-757 pubmed publisher
Kim M, Vargas M, Harlan B, Killoy K, Ball L, Comte Walters S, et al. Nitration and Glycation Turn Mature NGF into a Toxic Factor for Motor Neurons: A Role for p75NTR and RAGE Signaling in ALS. Antioxid Redox Signal. 2018;28:1587-1602 pubmed publisher
Mesentier Louro L, De Nicoló S, Rosso P, De Vitis L, Castoldi V, Leocani L, et al. Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells. Int J Mol Sci. 2017;18: pubmed publisher

image

image 1 :

Western blot analysis of Recombinant mouse proNGF protein (#N-250), (lanes 1-3) and Recombinant human proNGF protein (#N-280), (lanes 4-6): - 1,4. 100 ng + Anti-proNGF Antibody (ANT-005), (1:200).2,5. 250 ng + Anti-proNGF Antibody (1:200).3,6. 500 ng + Anti-proNGF Antibody (1:200).

image 2 :

Expression of proNGF in rat brain sections - Immunohistochemical staining of proNGF in rat brain sections using Anti-proNGF Antibody (#ANT-005). A. Staining of astrocytes was performed using mouse anti-glial fibrillary acidic protein (GFAP) (red). B. Co-localization between GFAP and proNGF (orange). C. Staining of proNGF (green).

image 3 :

Western blot analysisof mouse submandibular glands (SMGs): - 1.Anti-proNGF Antibody (#ANT-005)(1:200).2.Anti-proNGF Antibody preincubated with the control peptide antigen.

product information

CAT :

ANT-005

SKU :

ANT-005-CF_0.2 ml

Product Name :

Anti-proNGF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25427

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT005

Homology :

Mouse - identical; human - 16/21 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Pro-domain of the NGF protein

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)

Product Page - Scientific background :

Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Nerve growth factor precursor

Short Description :

A Rabbit Polyclonal Antibody to proNGF

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against rat proNGF. Anti-proNGF Antibody (#ANT-005) can be used in western blot and immunohistochemical applications. The antibody specifically reacts with proNGF. It has been designed to recognize proNGF from mouse, rat and human samples. Related products for neutralizing proNGF activity: Mouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Negative Control :

BLP-NT005

Positive Control :

Synonyms :

Nerve growth factor precursor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGF

AB Specifictiy :

The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage