Guinea pig Anti-proNGF Antibody | Alomone Labs ANT-005-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-proNGF Antibody

catalog :

ANT-005-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Mysona B, Zhao J, Smith S, Bollinger K. Relationship between Sigma-1 receptor and BDNF in the visual system. Exp Eye Res. 2018;167:25-30 pubmed publisher

image

image 1 :

Western blot analysis of 100 ng Recombinant mouse proNGF protein (#N-250) (lanes 1 and 3) and Recombinant human proNGF protein (#N-280) (lanes 2 and 4): - 1-2. Guinea pig Anti-proNGF Antibody (#ANT-005-GP), (1:200).3-4. Guinea pig Anti-proNGF Antibody, preincubated with the blocking peptide.

image 2 :

Expression of proNGF in mouse cortex - Immunohistochemical staining of perfusion-fixed mouse brain frozen sections using Guinea pig Anti-proNGF Antibody (#ANT-005-GP), (1:400), followed by goat-anti-guinea pig-Cy3. proNGF staining (red) appears in the pyramidal layer (arrows). Nuclei were stained with DAPI (blue).

product information

CAT :

ANT-005-GP

SKU :

ANT-005-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-proNGF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25427

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-NT005

Homology :

Mouse - identical; human - 16/21 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Pro-domain of the NGF protein

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)

Product Page - Scientific background :

Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Nerve growth factor precursor

Short Description :

A Guinea Pig Polyclonal Antibody to proNGF

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against rat proNGF. Guinea pig Anti-proNGF Antibody (#ANT-005-GP, formerly AGP-031) can be used in western blot and immunohistochemistry applications. Anti-proNGF Antibody raised in guinea pig recognizes the same epitope as Anti-proNGF Antibody (#ANT-005) raised in rabbit. The antibody specifically reacts with proNGF and does not crossreact with proBDNF, proNT-3 or mature NGF. It has been designed to recognize proNGF from mouse, rat and human samples. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc. Related products for neutralizing proNGF activity: Mouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Negative Control :

BLP-NT005

Positive Control :

Synonyms :

Nerve growth factor precursor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGF

AB Specifictiy :

The antibody is specific for proNGF; it does not crossreact with proBDNF, proNT-3 or mature NGF.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage