Anti-Neurotrophin 4 (NT-4) Antibody | Alomone Labs ANT-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurotrophin 4 (NT-4) Antibody

catalog :

ANT-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Li Q, Aalling N, Förstera B, Erturk A, Nedergaard M, Møllgård K, et al. Aquaporin 1 and the Na+/K+/2Cl- cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system. Fluids Barriers CNS. 2020;17:15 pubmed publisher

image

image 1 :

Western blot analysis of Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (10 ng): - 1. Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004), (1:200).2. Anti-Neurotrophin 4 (NT-4) Antibody, preincubated with Neurotrophin 4/NT-4 Blocking Peptide (#BLP-NT004).

image 2 :

Expression of Neurotrophin 4 in mouse spinal cord - Immunohistochemical staining of mouse spinal cord using Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004). The spinal ventral horn (VH) was very lightly stained. However, in the perimeter of the white matter (WM) there were NT-4 immunoreactive cells with a small soma (triangles) and some had a stained process (arrows).

image 3 :

Western blot analysisof Recombinant human Neurotrophin-4 (NT-4) protein (#N-270) (10 ng): - 1.Anti-Neurotrophin 4 (NT-4) Antibody(#ANT-004) (1:200).2. Anti-Neurotrophin 4 (NT-4) Antibody preincubated with the negative control antigen.

product information

CAT :

ANT-004

SKU :

ANT-004-CF_0.2 ml

Product Name :

Anti-Neurotrophin 4 (NT-4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P34130

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT004

Homology :

Rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4 (residues 123-132 of the precursor NT-4)

Product Page - Scientific background :

The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 Neurotrophin-4 (NT-4) is expressed in neurons of the superior cervical, stellate and celiac ganglion,2 T-cells3 and is synthesized by keratinocytes.4 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.5 The rat and human forms of NT-4 are 96% homologous. NT-4 has been shown to promote dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,6 to promote growth and remodeling of adult motor neuron innervation,7 to be anterograde survival factors for postsynaptic cells8 and to protect against apoptotic neuronal death.9 The biological effects of NT-4 are mediated by two receptors: TrkB, which is specific for NT-4 and BDNF, and p75NTR, which binds all the neurotrophins.10

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

NTF4, Neutrophic factor 4, Neurotrophin 5, NT-5, NTF5, Neutrophic factor 5, NT 4/5

Short Description :

A Rabbit Polyclonal Antibody to Neurotrophin 4

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Neurotrophin 4. Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004) can be used in western blot and immunohistochemistry applications. It has been designed to recognize NT-4 from rat, mouse and human samples.

Negative Control :

BLP-NT004

Positive Control :

Synonyms :

NTF4, Neutrophic factor 4, Neurotrophin 5, NT-5, NTF5, Neutrophic factor 5, NT 4/5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NTF4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage