Anti-Neurotrophin 3 (NT-3) Antibody | Alomone Labs ANT-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurotrophin 3 (NT-3) Antibody

catalog :

ANT-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Lee H, Jung D, Kim N, Shin H, Choi B. Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke. Neural Regen Res. 2022;17:1556-1565 pubmed publisher
Rodrigues Amorim D, Iglesias Martínez Almeida M, Rivera Baltanas T, Fernández Palleiro P, Freiría Martínez L, Rodríguez Jamardo C, et al. The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia. Int J Mol Sci. 2021;22: pubmed publisher
Jung D, Ahn S, Pak M, Lee H, Jung Y, Kim K, et al. Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD. elife. 2020;9: pubmed publisher
Kumar H, Lim C, Choi H, Joshi H, Kim K, Kim Y, et al. Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury. J Neurosci. 2020;40:1943-1955 pubmed publisher
Maimon R, Ionescu A, Bonnie A, Sweetat S, Wald Altman S, Inbar S, et al. miR126-5p Downregulation Facilitates Axon Degeneration and NMJ Disruption via a Non-Cell-Autonomous Mechanism in ALS. J Neurosci. 2018;38:5478-5494 pubmed publisher

image

image 1 :

Western blot analysis of Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (100 ng): - 1. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:200) 2. Anti-Neurotrophin 3 (NT-3) Antibody, preincubated with Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003).

image 2 :

Expression of Neurotrophin 3 in mouse spinal cord - Immunohistochemical staining of perfusion-fixed frozen mouse spinal cord sections with Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 (green). The same sections were stained with mouse anti-neurofilament 200 (NF) (red). Merge of the two images demonstrates the presence of NT-3 in gray matter which colocalizes with NF only in the soma of some neurons (arrow).

product information

CAT :

ANT-003

SKU :

ANT-003-CF_0.2 ml

Product Name :

Anti-Neurotrophin 3 (NT-3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P20783

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT003

Homology :

Rat, mouse, chicken, Xenopus - identical; cat - 9/10 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor)

Product Page - Scientific background :

The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.2 The rat and human forms of neurotrophin-3 (NT-3) are 96% homologous. NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,3 to serve as an anti-inflammatory factor, to suppress microglial activation,4 to play a critical role in regulating T helper 1/T helper 2 cell balance5 and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.6 The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3, and p75NTR, which binds all the neurotrophins.7

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

NTF3, Nerve growth factor 2, NGF2, HDNF

Short Description :

A Rabbit Polyclonal Antibody to Neurotrophin 3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Neurotrophin 3. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003) can be used in western blot and immunohistochemical applications. It has been designed to recognize NT-3 from rat, mouse and human samples.

Negative Control :

BLP-NT003

Positive Control :

Synonyms :

NTF3, Nerve growth factor 2, NGF2, HDNF

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NTF3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage