Anti-SLIT1 Antibody | Alomone Labs ANR-221 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLIT1 Antibody

catalog :

ANR-221

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human Jurkat T cell leukemia cell line lysate (lanes 3 and 6):

image 3 :

Expression of SLIT1 in mouse cerebellum.

product information

CAT :

ANR-221

SKU :

ANR-221-CF_0.2 ml

Product Name :

Anti-SLIT1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80TR4

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR221

Homology :

Human, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Secreted

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RDTYLQFTDLQNWPR, corresponding to amino acid residues 1170 - 1184 of mouse SLIT1

Product Page - Scientific background :

Slit homolog 1 protein (SLIT1), also known as multiple EGF-like domains protein 4, and MEGF4, is a secreted protein member of the SLIT family involved in axon guidance and cell migration.SLIT1 is a member of the SLIT family proteins that includes three members: SLIT1, SLIT2 and SLIT3. SLITS are secreted glycoproteins comprised of four stretches of leucine-rich repeat (LRR) domains, nine epidermal growth factor (EGF) repeats, a Laminin-G-like domain, and a C-terminal cysteine knot.1,2SLITS exert their many physiological roles by binding to Roundabout (Robo) receptors family, that includes four members (Robo 1 to Robo 4), which are single transmembrane receptors belonging to the immunoglobulin superfamily.1,2SLITS play a crucial role in axon guidance during the development of the nervous system. Axon guidance is the process by which neurons extend their axons to reach their appropriate targets, such as other neurons, muscles, or glands. This process is essential for the formation of functional neural circuits.1,3In addition, SLIT1 is involved in various aspects of the regulation of neural progenitor cells (NPCs). NPCs are the precursors to neurons and glial cells in the nervous system. They undergo proliferation, migration, and differentiation to generate the diverse cell types found in the mature brain. SLIT1 is involved in several aspects of NPCs functions, including promoting the proliferation of NPCs in several brain regions, guiding NPCs from the sub-ventricular zone towards the cortical plate and supporting the differentiation of NPCs into specific neuronal or glial cell types.3,4Finally, a complex role for SLIT1 in cancer has emerged. SLIT1 has been ascribed a tumor suppressive role in certain cancers such as breast and colorectal, while in others it appears to associate with tumor progression. In particular, in glioma and pancreatic cancer, SLIT1 expression has been correlated with enhanced metastasis formation and poor prognosis. 2,5,6

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Slit Guidance Ligand 1, Slit Homolog 1 Protein, Multiple EGF-Like Domains Protein 4, MEGF4

Short Description :

A Rabbit Polyclonal antibody to SLIT1

Long Description :

Anti-SLIT1 Antibody (#ANR-221) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLIT1 from rat, mouse and human samples.

Negative Control :

BLP-NR221

Positive Control :

Synonyms :

Slit Guidance Ligand 1, Slit Homolog 1 Protein, Multiple EGF-Like Domains Protein 4, MEGF4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLIT1

AB Specifictiy :

This antibody is specific to SLIT1, it won’t recognize the highly homologous protein SLIT2.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage