Anti-PTGFRN (extracellular) Antibody | Alomone Labs ANR-212 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PTGFRN (extracellular) Antibody

catalog :

ANR-212

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):

image 2 :

Western blot analysis of human pancreatic carcinoma PANC-1 cell line lysate (lanes 1 and 4), human breast adenocarcinoma MCF-7 cell line (lanes 2 and 5) and human U-87 MG glioblastoma cell line (lanes 3 and 6):

image 3 :

Expression of PTGFRN in mouse hypothalamus:

product information

CAT :

ANR-212

SKU :

ANR-212-CF_0.2 ml

Product Name :

Anti-PTGFRN (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WV91

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR212

Homology :

Rat – identical Human – 14 out of 15 amino acid identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EDEFRYRMYQTQVSD, corresponding to amino acid residues 635 - 649 of mouse PTGFRN

Product Page - Scientific background :

The Prostaglandin F2 Receptor Negative Regulator (PTGFRN), also known as EWI-F and CD9P-1, is a member of the immunoglobulin (Ig) superfamily. PTGFRN is a type I single-pass transmembrane protein and part of the cell adhesion molecule (CAM) family, which plays essential roles in cell-cell and cell-matrix interactions1,2. CAMs, including PTGFRN, are characterized by extracellular Ig-like domains and contribute to cellular adhesion, migration, and signaling. PTGFRN interacts with tetraspanins (CD9, CD81), integrins, and ERM proteins, functioning as a scaffold in tetraspanin-enriched microdomains (TEMs)3. These interactions enable its role in tumor progression, metastasis, and angiogenesis by regulating PI3K/AKT and mTOR signaling pathways1,2. PTGFRN is highly expressed in glioblastoma (GBM) and several cancers, with significantly elevated levels in tumor tissues compared to normal tissues1,2,3. It is implicated in tumor proliferation, migration, and radiation resistance. In neurodegenerative disorders, PTGFRN correlates with Alzheimer’s disease progression via its role in extracellular vesicles (EVs) associated with amyloid plaques3. PTGFRN’s association with poor prognosis in cancers and its involvement in neurodegeneration highlight its potential as a therapeutic target. Immunodetection of PTGFRN can benefit research areas in oncology, neurobiology, and regenerative medicine1,2,4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Prostaglandin F2 Receptor Inhibitor, CD9 Partner 1, CD9P-1, FPRP, Glu-Trp-Ile EWI motif-containing protein F, EWI-F, CD315

Short Description :

A Rabbit polyclonal antibody to PTGFRN (extracellular)

Long Description :

Anti-PTGFRN (extracellular) Antibody (#ANR-212) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PTGFRN from mouse, rat and human samples.

Negative Control :

BLP-NR212

Positive Control :

Synonyms :

Prostaglandin F2 Receptor Inhibitor, CD9 Partner 1, CD9P-1, FPRP, Glu-Trp-Ile EWI motif-containing protein F, EWI-F, CD315

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTGFRN

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage