Anti-CD81 (extracellular)-PE Antibody | Alomone Labs ANR-211-PE product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CD81 (extracellular)-PE Antibody

catalog :

ANR-211-PE

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of CD81 by direct flow cytometry in live intact mouse BV-2 microglia cell line:

image 2 :

Cell surface detection of CD81 by direct flow cytometry in live intact human U-87 MG glioblastoma cell line:

image 3 :

Cell surface detection of CD81 by direct flow cytometry in live intact intact U87- derived extracellular vesicles (EVs):

product information

CAT :

ANR-211-PE

SKU :

ANR-211-PE_15 mcl

Product Name :

Anti-CD81 (extracellular)-PE Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P60033

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR211

Homology :

Mouse, rat - 11 out of 12 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, 2nd loop.

Label :

R-Phycoerythrin (R-PE).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CHQKIDDLFSGK, corresponding to amino acid residues 190 - 201 of human CD81

Product Page - Scientific background :

Cluster of differentiation 81, CD81, also known as Tetraspanin-28, Tspan28, and target of the antiproliferative antibody 1, TAPA-1, is a transmembrane (TM) protein, member of the tetraspanin family, and plays a role in intercellular interactions and cellular trafficking.1CD81 is composed of 236 amino acids, four TM regions, intracellular N- and C-termini, small (EC1) and large (EC2) extracellular loops, a short intracellular loop between TM2 and TM3, and is characterized by a bound cholesterol molecule in an intramembrane pocket between the two pairs of TM helices capped by the EC2 domain. The binding of cholesterol alters the conformation of the protein, a process which is exploited by certain viruses to enter cells. CD81 serves as a co-receptor for several viruses, including hepatitis C (HCV), human immunodeficiency virus type 1 (HIV-1), herpes simplex virus 1 (HSV-1), influenza A (IAV), and Chikungunya, as well as certain bacteria and parasites. CD81 can interact with other TM proteins to form clusters and higher-order protein complexes.1,2CD81 is highly conserved in mammals, expressed on all primary B-lineage cells from early B-cell development stages, and associates with CD19 on the cell surface. CD81 engagement on mature human B-cells induces an antiproliferative effect.3CD81 is involved in neurotransmission, cell signaling, cell proliferation, and protein trafficking, as well as the immune, reproductive, genitourinary, and auditory systems. CD81 is also implicated in oncology, viral infection, and inflammation.1,2

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1

Short Description :

A Rabbit Polyclonal antibody to CD81 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).

Long Description :

Anti-CD81 (extracellular) Antibody (#ANR-211) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD81 from mouse, rat and human samples. Anti-CD81 (extracellular)-PE Antibody (#ANR-211-PE) is directly conjugated to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-PE

Positive Control :

Synonyms :

Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CD81

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANR-211), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage