antibody

Anti-CD63 (extracellular) Antibody

ANR-210

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

western blot, immunohistochemistry

ANR-210-CF_0.2 ml

Anti-CD63 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P41731

IFC IHC LCI WB

Rat Mouse

Rabbit

BLP-NR210

Rat - identical Won't recognize CD63 from human samples

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, 2nd loop.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

CGNDFKESTIHTQG, corresponding to amino acid residues 177 - 190 of mouse CD63

CD63, also known as Tetraspanin-30 (Tspan-30), lysosomal-associated membrane protein 3 (LAMP-3), lysosome integral membrane protein 1 (Limp1), melanoma-associated antigen (ME491), and ocular melanoma-associated antigen (OMA81H), is a multi-transmembrane protein that plays a role in cancer malignancy.1CD63 is a highly N-glycosylated member of the transmembrane-4 superfamily of tetraspanin proteins. CD63 is composed of 238 amino acids comprising four transmembrane α-helices, two extracellular loops, and a short cytoplasmic tail. CD63 is located in late endosomes, lysosomes, secretory vesicles, and the plasma membrane. At the plasma membrane, CD63 interacts with cell adhesion molecules such as integrins in order to regulate intracellular signaling pathways for cell adhesion, motility, and survival. CD63 is also involved in membrane transport, fusion, and protein kinase signaling.1,2In addition to its role as a confirmed marker for cancer, the significance of CD63 in identifying exosomes has recently been highlighted. Exosomes are small extracellular vesicles, ranging from 50 to 150 nm in diameter, characterized by a lipid membrane structure. They are widely present in bodily fluids and can be generated by almost all cell types, whether in pathological or physiological conditions. Importantly, the contents of exosomes are carefully regulated by their originating cells, allowing them to convey specific information from the parent cells to other cells. This enables the assessment of the functional states of specific cells by examining the exosomes they release, paving the way for non-invasive cancer liquid biopsies facilitated by exosomes.1The presence of specific protein markers on exosomes has been observed, opening up possibilities for the development of an immunoaffinity-based strategy to separate exosomes. This strategy involves exploring the specific binding between these markers and their corresponding affinity agents, such as antibodies. The landscape of present-day biological research is undergoing a significant transformation due to the advancements in exosome-mediated therapeutics and diagnosis. Consequently, CD63 emerges as a highly promising target, not only for evaluating cancer, but also for delivering drugs with precision to tumor cells that express CD63. This targeted approach holds great potential for precise anti-cancer therapy.1

IFC IHC LCI WB

Alomone Labs

Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491

A Rabbit Polyclonal antibody to CD63 (extracellular)

Anti-CD63 (extracellular) Antibody (#ANR-210) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD63 from mouse and rat samples. The antibody will not work with human samples.

BLP-NR210

NA

Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

CD63

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:200-1:500