Anti-CD9 (extracellular) Antibody | Alomone Labs ANR-209 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CD9 (extracellular) Antibody

catalog :

ANR-209

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of rat lung lysate:

image 3 :

Western blot analysis of human THP-1 monocytic leukemia cell line lysates (lanes 1 and 4), human MEG-01 megakaryoblast cell line lysate (lanes 2 and 5) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 6):

product information

CAT :

ANR-209

SKU :

ANR-209-CF_0.2 ml

Product Name :

Anti-CD9 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P40240

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR209

Homology :

Rat - identical, Human - 14 out of 16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EVIKELQEFYKDTYQK, corresponding to amino acid residues 114 - 129 of mouse CD9

Product Page - Scientific background :

Cluster of Differentiation 9 (CD9), also known as Tetrapanin-29, TSPAN29 and Leukocyte Antigen MIC3, is a member of the tetraspanin superfamily, a group of proteins characterized by four transmembrane domains, two extracellular loops, and N- and C- termini cytoplasmic tails.1The tetraspanins family members function as organizers of the cell surface by recruiting specific partner proteins into tetraspanin-enriched microdomains. Thus, depending on the recruited protein, they influence many cellular activities in different cells, including intracellular signaling, proliferation, activation, survival, migration, invasion, adhesion, and diapedesis.1CD9 was originally identified as a lymphohematopoietic marker, and is indeed expressed in all the major subsets of leukocytes, however, it is also widely expressed across different cell populations including endothelial cells, neurons, glia and oocytes.2,3CD9 plays various roles in the immune system, it has been implicated in T cell activation and participating in the formation of immunological synapses by interacting with the integrin LFA-1 (on the T-cell side) and their ligand, the integrin ICAM-1 on the antigen presenting cell (APC) surface.2,3 In B cells, CD9 plays a role in B cell activation and regulation of antibody production, and in mast cells is involved in the regulation of signalling pathways that influence the release of allergic mediators in response to allergens.2,3CD9 has complex roles in the central nervous system (CNS). It has been implicated in neurite outgrowth and cell migration in association with the neural adhesion molecule L1 (L1CAM). It is also involved in myelination and the maintaining of the myelin sheath, as well as the regulation of neural progenitor cells.4Finally, CD9 is often associated with exosomes, small extracellular vesicles released by cells that play a crucial role in intercellular communication by carrying proteins, lipids, and nucleic acids between cells. CD9 is considered a marker for exosomes and detection of CD9 on the surface of extracellular vesicles is often used as a method to isolate and identify exosomes from biological fluids or cell culture supernatants.5,6

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3

Short Description :

A Rabbit Polyclonal antibody to CD9 (extracellular)

Long Description :

Anti-CD9 (extracellular) Antibody (#ANR-209) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD9 from mouse, rat and human samples.

Negative Control :

BLP-NR209

Positive Control :

Synonyms :

Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CD9

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage