Anti-IGSF8 (extracellular) Antibody | Alomone Labs ANR-208 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-IGSF8 (extracellular) Antibody

catalog :

ANR-208

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human Burkitt's lymphoma (Daudi) cell line lysate (lanes 2 and 6), human normal skin fibroblast Malme-3 cell line lysate (lanes 3 and 7) and Human prostate carcinoma LN-CaP cell line lysate (lanes 4 and 8):

image 3 :

Cell surface detection of IGSF8 by indirect flow cytometry in live intact mouse J774 macrophage cell line:

product information

CAT :

ANR-208

SKU :

ANR-208-CF_0.2 ml

Product Name :

Anti-IGSF8 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8R366

Applications :

FC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR208

Homology :

Human–15/16 identical, Rat–14/16 identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EAGAPYAERLASGELR, corresponding to amino acid residues 227 - 242 of mouse IGSF8

Product Page - Scientific background :

IGSF8, also referred to as EWI-2, CD316, and PGRL, is a member of the immunoglobulin superfamily (IgSF), which is characterized by extracellular immunoglobulin-like domains. IGSF8 specifically contains four such domains along with a short cytoplasmic tail, enabling its roles in cell-cell adhesion, intracellular signaling, and the organization of cell surface microdomains1,2. It associates with tetraspanins, such as CD9 and CD81, forming the tetraspanin web, a molecular network that organizes cell-surface proteins into functional microdomains and regulates processes like adhesion, migration, and signaling3. Furthermore, IGSF8 interacts with the ezrin-radixin-moesin (ERM) protein family, linking the tetraspanin web to the actin cytoskeleton and contributing to cell polarity and motility4,5. Functionally, IGSF8 acts as an immune checkpoint by suppressing natural killer (NK) cell cytotoxicity through interactions with receptors like KIR3DL2, allowing tumor cells to evade immune detection3. Its expression correlates with poor outcomes in cancer and low antigen presentation, making it a promising target for therapeutic intervention3,4. Beyond its role in immune modulation, IGSF8 contributes to reproductive biology by participating in sperm-egg fusion2. IGSF8 is expressed across various tissues, including neural and immune cells1. Disruption of its function enhances cellular migration and inhibits metastasis4, highlighting its therapeutic relevance. As a cancer immunotherapy target, IGSF8 has shown potential, particularly when combined with immune checkpoint inhibitors like anti-PD1 antibodies3. Research into IGSF8 is critical for understanding tumor immune evasion, immunotherapeutic development, and its roles in neural and reproductive biology1,5.

Applications may also work in :

FC LCI WB

Supplier :

Alomone Labs

Target :

Immunoglobulin superfamily member 8, Prostaglandin Regulatory-Like Protein, PGRL, Glu-Trp-Ile EWI motif-containing protein 2, EWI2, CD81 Partner 3, KCT4, CD316

Short Description :

A Rabbit polyclonal antibody to IGSF8 (extracellular)

Long Description :

Anti-IGSF8 (extracellular) Antibody (#ANR-208) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize IGSF8 from mouse, rat and human samples.

Negative Control :

BLP-NR208

Positive Control :

Synonyms :

Immunoglobulin superfamily member 8, Prostaglandin Regulatory-Like Protein, PGRL, Glu-Trp-Ile EWI motif-containing protein 2, EWI2, CD81 Partner 3, KCT4, CD316

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

IGSF8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage