Anti-Neogenin (extracellular) Antibody | Alomone Labs ANR-195 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neogenin (extracellular) Antibody

catalog :

ANR-195

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat spleen membranes (lanes 2 and 4): - 1-2. Anti-Neogenin (extracellular) Antibody (#ANR-195), (1:200). 3-4. Anti-Neogenin (extracellular) Antibody, preincubated with Neogenin (extracellular) Blocking Peptide (BLP-NR195).

image 2 :

Expression of Neogenin in rat parietal cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neogenin (extracellular) Antibody (#ANR-195), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Neogenin immunoreactivity (green) appears in cortical neurons in layer 3 (arrows). B. Pre-incubation of the antibody with Neogenin (extracellular) Blocking Peptide (BLP-NR195), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-195

SKU :

ANR-195-CF_0.2 ml

Product Name :

Anti-Neogenin (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97798

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR195

Homology :

Human, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-term.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KIRYRKASRKSDVTE, corresponding to amino acid residues 701 - 715 of mouse Neogenin

Product Page - Scientific background :

Neogenin also known as NEO1 is a member of the immunoglobulin (Ig) superfamily. It has been reported to be involved in diverse physiology and pathology functions, including cell proliferation, differentiation and migration1. Neogenin was originally isolated from embryonic chicken cerebellum and reported to be homologous to the axon guidance receptor deleted in colorectal cancer (DCC)2.Neogenin consists of 4 N-terminal Ig-like domains, followed by 6 fibronectin type III-like domains, a single transmembrane helix, and an intracellular domain. Neogenin is capable to mediate attractive and repulsive axon guidance depending on the ligand identity. Two main ligands of neogenin are Netrin-1 (NET1) and repulsive guidance molecules (RGMs)3.Interaction of Neogenin with RGMs leads to cytoskeleton rearrangements via Rho GTPases, this results in growth cone collapse. In contrast interaction with Netrin-1 triggers attractive growth cone response3. Recently it was shown that Neogenin plays a role in the maintenance of iron homeostasis via interaction with HJV (members of the RGM family)4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Neogenin 1, NEO1, NGN

Short Description :

A Rabbit Polyclonal antibody to Neogenin (extracellular)

Long Description :

Anti-Neogenin (extracellular) Antibody (#ANR-195) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Neogenin from rat, mouse and human samples.

Negative Control :

BLP-NR195

Positive Control :

Synonyms :

Neogenin 1, NEO1, NGN

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NEO1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage