Anti-Tetraspanin 2 (extracellular) Antibody | Alomone Labs ANR-188 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Tetraspanin 2 (extracellular) Antibody

catalog :

ANR-188

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and mouse P815 mastocytoma cell line lysate (lanes 2 and 4):

image 3 :

Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):

product information

CAT :

ANR-188

SKU :

ANR-188-CF_0.2 ml

Product Name :

Anti-Tetraspanin 2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q922J6

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR188

Homology :

Rat - identical, Human - 14 out of 17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)DVAIRHVQSMYEEAYSD, corresponding to amino acid residues 114 - 130 of mouse TSPAN2

Product Page - Scientific background :

Tetraspanin 2 (TSPAN2) is a member of the Tetraspanin family of proteins, that comprises 33 members in humans. The family is characterized by the presence of four transmembrane domains, which give rise to the name "tetraspanin", as well as the presence two extracellular loops (EC1 and EC2), a small cytoplasmic loop, and short N- and C-terminal tails 1,2.Tetraspanins are able to interact with each other and also with numerous partner proteins such as integrins, growth factor receptors and ion channels, to create a dynamic network of interactions known as the tetraspanin-enriched microdomains (TEMs). Consequently, tetraspanins are regarded as molecular organizers and by forming complexes with these proteins, tetraspanins can modulate signal transduction pathways and cellular responses 2,3,4.Although TSPAN2 shares a relatively high homology with well-studied tetraspanins like CD9 (TSPAN29) or CD81 (TSPAN28), it has been less well-characterised up until now 3.Nevertheless, TSPAN2, like the related CD9 and CD81 proteins, has been described in relation to exosome formation, specifically regarding neuroinflammation and microglia activation 5, as well as oligodendrocyte differentiation into myelin-forming glia and in the progression of tumor metastasis by modulating cancer cell motility and invasion 3,5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Tetraspan NET-3, TSPAN2

Short Description :

A Rabbit Polyclonal antibody to Tetraspanin 2 (extracellular)

Long Description :

Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize TSPAN2 from mouse, rat and human samples.

Negative Control :

BLP-NR188

Positive Control :

Synonyms :

Tetraspan NET-3, TSPAN2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TSPAN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage