Anti-Tetraspanin 7 (extracellular) Antibody | Alomone Labs ANR-187 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Tetraspanin 7 (extracellular) Antibody

catalog :

ANR-187

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:500). 3-4. Anti-Tetraspanin 7 (extracellular) Antibody, preincubated with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187).

image 2 :

Expression of Tetraspanin 7 in the rat substantia nigra region. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Tetraspanin 7 immunoreactivity (green) appears in neuronal profiles (arrows) in both pars reticulata (SNR) and pars compacta (SNC). B. Pre-incubation of the antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-187

SKU :

ANR-187-CF_0.2 ml

Product Name :

Anti-Tetraspanin 7 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41732

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR187

Homology :

Mouse - 15 out of 16 amino acid residues identical; rat - 14 out of 16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DWENTDWFKETKNQS, corresponding to amino acid residues 155 - 169 of human Tetraspanin-3

Product Page - Scientific background :

Tetraspanins (Tspans) are a family of four-span transmembrane proteins, known as plasma membrane "master organizers." They form Tspan-enriched microdomains (TEMs or TERMs) through lateral association with one another and other membrane proteins.1Tetraspanins are characterized by four transmembrane domains, two extracellular loops: one small extracellular loop (SEL or EC1) and one larger extracellular loop (LEL or EC2), a short intracellular loop, and two short intracellular tails.1Teatraspanin 7 (TSPAN7) is highly expressed in the brain and was first described as a candidate gene for X-linked intellectual disability, probably through the modulation of surface expression of AMPA and dopamine receptors.2Tetraspanin 7 also has a key role in controlling pancreatic β-cell Ca2+ handling and defining the set-point of insulin secretion through modulation of L-type Cav1.2 and Cav1.3 channels. Furthermore, it has been identified as a target for autoantibodies in Type I diabetes. 3In addition, Tetraspanin 7 is involved in the regulation of HIV virus entry into dendritic cells and several types of cancer progression. 2

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.

Short Description :

A Rabbit Polyclonal Antibody to Tetraspanin 7 (extracellular)

Long Description :

Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187) is a highly specific antibody directed against an extracellular epitope of human Tetraspanin 7. The antibody can be used in western blot, indirect flow cytometry and immunohistochemistry. It has been designed to recognize Tetraspanin 7 from mouse, rat and human samples.

Negative Control :

BLP-NR187

Positive Control :

Synonyms :

TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TSPAN7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400-1:500

more info or order :

Alomone Labs product webpage