Anti-Tetraspanin-3 (extracellular) Antibody | Alomone Labs ANR-185 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Tetraspanin-3 (extracellular) Antibody

catalog :

ANR-185

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1-2. Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:400). 3-4. Anti- Tetraspanin-3 (extracellular) Antibody, preincubated with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185).

image 2 :

Expression of Tetraspanin-3 in rat cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:300), followed by donkey anti-rabbit-biotin and streptavidin-Cy3. A. Tetraspanin-3 immunoreactivity (red) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-185

SKU :

ANR-185-CF_0.2 ml

Product Name :

Anti-Tetraspanin-3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O60637

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR185

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DWENTDWFKETKNQS, corresponding to amino acid residues 155 - 169 of human Tetraspanin-3

Product Page - Scientific background :

Tetraspanin-3, encoded by TSPAN3 gene is a membrane-spanning glycoprotein that has a role in cell migration, proliferation, and interactions between cells and extracellular matrix.As suggested by the family name, TSPANs are categorized by the presence of four trans-membrane domains, of which two are extracellular. The intracellular part includes the C and N terminal domains that can facilitate protein-protein interactions, as well as guide intracellular trafficking. TSPANs have multiple roles In the immune and vascular systems, and they act as organizers of functional microdomains and multi-subunit receptor complexes 1.Tspan3 is highly expressed in the brain and elevated in brains of Alzheimers disease (AD) patients. It was shown to interact with ADAM10, which is a marker for Alzheimer's disease 2. It was also shown to be expressed by dendritic cells (DCs) which suggests that it may be involved in the function of DCs in association with T cell stimulation 3.Its functional diversity and importance for the development and onset of various diseases makes Tetraspanin 3 an appealing research target.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8

Short Description :

A Rabbit Polyclonal antibody to Tetraspanin-3 (extracellular)

Long Description :

Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Tetraspanin-3 from rat, mouse and human samples.

Negative Control :

BLP-NR185

Positive Control :

Synonyms :

TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TSPAN3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage