Anti-Robo2 (extracellular) Antibody | Alomone Labs ANR-182 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Robo2 (extracellular) Antibody

catalog :

ANR-182

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of human lung carcinoma NCI-H526 cell line lysate (lanes 1 and 3) and human chronic myelogenous leukemia K562 cell line lysate (lanes 2 and 4):

image 3 :

Cell surface detection of Robo2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:

product information

CAT :

ANR-182

SKU :

ANR-182-CF_0.2 ml

Product Name :

Anti-Robo2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7TPD3

Applications :

IFC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR182

Homology :

Human, rat - identical.

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KVPTERSAVLVNLKK, corresponding to amino acid residues 694 - 708 of mouse Robo2

Product Page - Scientific background :

Robo2 (Roundabout homolog 2) is a member of the immunoglobulin superfamily and part of the ROBO receptor family, which is critical in the SLIT-ROBO signaling pathway. This receptor, characterized by extracellular immunoglobulin (Ig) and fibronectin type III (FNIII) domains, a transmembrane region, and an intracellular domain, mediates cellular migration, adhesion, and tissue remodeling during development and adulthood1. Robo2 is expressed in the nervous system, kidneys, pancreas, lungs, and retinal vasculature, contributing to organogenesis, angiogenesis, and immune modulation2,3. Subcellularly, it localizes to the plasma membrane. In axonal guidance, SLIT2 binding activates Robo2, leading to cytoskeletal remodeling via Rho family GTPases, while in non-neuronal tissues, it engages TGF-β signaling to regulate mesenchymal activation and tissue fibrosis3,4. Dysregulation of Robo2 is implicated in several diseases. Mutations in ROBO2 are associated with congenital anomalies of the kidney and urinary tract (CAKUT), where disrupted SLIT-ROBO signaling impairs ureteric budding and nephric duct patterning1,4. In the pancreas, ROBO2 acts as a stroma suppressor, limiting TGF-β-driven myofibroblast activation and immune cell infiltration in pancreatic ductal adenocarcinoma (PDAC)2. In the retina, Robo2 promotes angiogenesis and vascular development, partly compensating for VEGF signaling³. Reduced expression of ROBO2 in PDAC and ocular diseases is correlated with poorer outcomes2,3,5. This receptor’s roles in axon guidance, organogenesis, and disease make it a significant target for therapeutic strategies, including inhibitors of SLIT-ROBO or TGF-β pathways2,3,5.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

Roundabout homolog 2, Roundabout Guidance Receptor 2

Short Description :

A Rabbit Polyclonal Antibody to Robo2

Long Description :

Anti-Robo2 (extracellular) Antibody (#ANR-182) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot application. It has been designed to recognize Robo2 from mouse, rat and human samples.

Negative Control :

BLP-NR182

Positive Control :

Synonyms :

Roundabout homolog 2, Roundabout Guidance Receptor 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

Robo2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Standard quality control of each lot :

Western blot analysis.

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage